General Information
- Sample ID
- OES334707
- Sample Name
- OES-CUi-Cui-2243122
- Subject type
- Cell line
Sample information
- Organism
- Cancer
- Tissue
- cell line
- Projects
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1
Project ID Project name Submitter Date OEP004750 EMSY enhances cancer stem cell self-renewal and tumorigenesis by reshaping methionine metabolism in triple-negative breast cancer Liu, Cui-Cui 2023-11-20 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX025490 OEX_Cui-Cui_2311201516 Genomic Briefly, 107 to 108 TNBC cells (Control or EMSY) were harvested, crosslinking was performed with 1% formaldehyde or 1% paraformaldehyde for 10 minutes. To enhance cell lysis, we ran the lysate through a 27g needle three times and flash froze it in −80°C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 20 minutes. Protein/DNA complex was precipitated by specific antibodies against H3K4me2/3 (CST) and IgG control (Millipore, Burlington, MA, USA). DNA was purified using DNA Purification Kit (Qiagen, Hilden, Germany). PCR was performed using Phusion High Fidelity PCR master mix. ChIP-enriched chromatins were sequenced on Illumina HiSeq 2000 platforms according to the manufacture’s protocol. 2023-11-20 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER440354 OER_Cui-Cui_2311201543 OEP004750 OEX025490 OES334707 1 2023-11-20
Author Information
- Create Date
- 2023-11-20
- Last Modified
- 2023-11-20
- Submission
- Cui-Cui Liu,Shanghai Cancer Center