Results of SAS Job ID: 20200630135440c2692d

1. Your queried sequence

>EPI_ISL_402132
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSITRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT

(Sequence length 1273 amino acids)

2. The antigenic similarity sores are illustrated as below*:

Pre-defined epitopes CE-BLAST score between your query and benchmarking SARS-CoV-2 (AAC)
spike_validated_epi_1 0.977
spike_validated_epi_2 0.815
spike_validated_epi_3 0.837
spike_validated_epi_4 0.755
spike_validated_epi_5 0.976
spike_validated_epi_6 0.978
spike_validated_epi_7 0.801
spike_validated_epi_8 0.770
spike_validated_epi_9 0.821
spike_validated_epi_10 0.691
spike_validated_epi_11 0.973
spike_validated_epi_12 0.965
spike_validated_epi_13 0.574
spike_validated_epi_14 0.691
spike_validated_epi_15 0.553
spike_predicted_epi_1 0.961
spike_predicted_epi_2 0.983
spike_predicted_epi_3 0.950
spike_predicted_epi_4 0.975
spike_predicted_epi_5 0.969
spike_predicted_epi_6 0.979
spike_predicted_epi_7 0.956
spike_predicted_epi_8 0.941
spike_predicted_epi_9 0.970
spike_predicted_epi_10 0.806
spike_predicted_epi_11 0.946
spike_predicted_epi_12 0.957
spike_predicted_epi_13 0.945

* The similarity score is calculated between query S protein and the S protein of the benchmarking SARS-CoV-2 through CE-BLAST [1]. The similarity score for the S protein of benchmarking SARS-CoV and BatCoV against benchmarking SARS-CoV-2 are also provided as reference.
** Suggested cutoff:
antigenic score > 0.8, antigenic similar;
0.7 < antigenic score < 0.8, marginally similar; (May or may not change);
0.6 < antigenic score < 0.7, slightly different (Moderately change);
antigenic score < 0.6, antigenic different (Significantly change).

Reference CE-BLAST score on each epitope.

Pre-defined epitopes CE-BLAST score between benchmarking SARS(AAA) and SARS-CoV-2 (AAC) CE-BLAST score between benchmarking BatCoV (AAB) and SARS-CoV-2 (AAC)
spike_validated_epi_1 0.664 0.806
spike_validated_epi_2 0.417 0.386
spike_validated_epi_3 0.455 0.758
spike_validated_epi_4 0.539 0.524
spike_validated_epi_5 0.507 0.922
spike_validated_epi_6 0.748 0.727
spike_validated_epi_7 0.531 0.544
spike_validated_epi_8 0.505 0.488
spike_validated_epi_9 0.417 0.278
spike_validated_epi_10 0.347 0.383
spike_validated_epi_11 0.662 0.709
spike_validated_epi_12 0.678 0.780
spike_validated_epi_13 0.374 0.366
spike_validated_epi_14 0.328 0.574
spike_validated_epi_15 0.182 0.325
spike_predicted_epi_1 0.709 0.905
spike_predicted_epi_2 0.748 0.980
spike_predicted_epi_3 0.563 0.902
spike_predicted_epi_4 0.702 0.983
spike_predicted_epi_5 0.549 0.875
spike_predicted_epi_6 0.574 0.984
spike_predicted_epi_7 0.784 0.944
spike_predicted_epi_8 0.592 0.867
spike_predicted_epi_9 0.644 0.972
spike_predicted_epi_10 0.615 0.865
spike_predicted_epi_11 0.366 0.914
spike_predicted_epi_12 0.448 0.937
spike_predicted_epi_13 0.450 0.899

3. Position of Epitope region:

spike_validated_epi_1:
372,375,376,378,403,404,405,408,437,439,440,449,496,498,499,500,501,502,503,504,505,506,508
spike_validated_epi_2:
446,449,484,485,486,489,490,492,493,494,496,498,501,505
spike_validated_epi_3:
403,405,409,415,416,417,420,421,453,455,456,457,458,460,473,475,476,477,486,487,489,490,493,495,496,498,500,501,502,505
spike_validated_epi_4:
403,405,408,415,416,417,421,453,455,456,457,458,459,460,473,474,475,476,477,486,487,489,498,500,501,502,504,505
spike_validated_epi_5:
369,370,371,372,374,376,377,378,379,380,381,382,383,384,385,386,390,392,428,429,430,515,516,517,518,519
spike_validated_epi_6:
334,335,337,339,340,343,344,345,346,354,356,357,359,360,361,441,509
spike_validated_epi_7:
403,405,406,408,409,415,416,417,420,421,455,456,457,458,460,473,474,475,476,477,486,487,489,493,495,502,504,505
spike_validated_epi_8:
449,453,455,456,483,484,485,486,487,489,490,492,493,494
spike_validated_epi_9:
346,444,446,447,448,449,450,452,483,484,485,490,494
spike_validated_epi_10:
417,453,455,456,484,485,486,487,488,489,493
spike_validated_epi_11:
369,374,375,376,377,378,379,380,381,382,383,384,385,386,388,389,390,392,411,412,413,414,427,428,429,430,517
spike_validated_epi_12:
369,372,373,374,375,376,377,378,379,380,385,405,407,408,411,412,503,504
spike_validated_epi_13:
446,449,456,475,483,484,485,486,487,489,493
spike_validated_epi_14:
144,145,146,147,150,152,245,246,247,248,249
spike_validated_epi_15:
444,446,447,449,450,452,470,478,479,481,482,483,484,485,490
spike_predicted_epi_1:
30,32,33,34,56,57,58,59,60,61,63,191,217,218,219,266,267,293
spike_predicted_epi_2:
38,39,40,41,42,43,44,45,49,51,52,53,204,206,220,221,222,224,225,278,279,280,282,283,284,285,286,287,288
spike_predicted_epi_3:
50,273,274,291,298,301,302,303,304,305
spike_predicted_epi_4:
65,80,81,82,83,84,85,86,104,108,110,199,231,233,234,235,236,237,238,239,269
spike_predicted_epi_5:
109,111,112,113,114,115,116,132,133,134,135,136,137,139
spike_predicted_epi_6:
327,328,329,388,389,528,529,530,531,532,533,534,542,544,545,546,547,578,579,580
spike_predicted_epi_7:
341,342,344,345,346,347,348,349,350,351,399,401,402,436,442,448,450,451,454,509
spike_predicted_epi_8:
600,601,603,604,605,663,673,674,675,676,689,690,691,692
spike_predicted_epi_9:
654,655,656,657,658,660,671,693,695,696
spike_predicted_epi_10:
659,661,662,669,697,698,699,700,701,702
spike_predicted_epi_11:
723,724,725,726,938,1028,1041,1042,1043,1044,1045,1047,1048,1068
spike_predicted_epi_12:
727,728,729,730,731,777,778,782,944,945,946,947,948,949,951,1017,1018,1019,1020,1021,1022,1023,1024,1026,1027,1056,1057,1058,1059
spike_predicted_epi_13:
884,885,886,887,888,889,890,891,892,893,894,895,1035

For more information, please contact Dr. Cao at zwcao@tongji.edu.cn

Reference

[1] Qiu T, Yang Y, Qiu J, et al. CE-BLAST makes it possible to compute antigenic similarity for newly emerging pathogens. Nat Commun. 2018;9(1):1772. Published 2018 May 2. doi:10.1038/s41467-018-04171-2