General information
Name
'Pseudoflavonifractor muris'
Nomenclature type
524-17T;CGMCC 1.31009
Etymology
mu’ris L. gen. n. muris of a mouse
Ecology
Isolation source
isolated from the cecal contents of an 8-week-old male Lepob/ob C57BL/6J mouse.
Morphology
The taxon 76, respresented by strain 524-17T, is phylogenetically closest to Pseudoflavonifractor phocaeensis30, with 16S rRNA gene identity of 95%. Phylogenetic tree shows that strain 524-17T clusters with other members of the genus Pseudoflavonifractor (Figure SF-43a), suggesting strain 524-17T is a member of the genus Pseudoflavonifractor. The genome of strain 524-17T was sequenced and the GenBank accession number is QWEK00000000. Genome-based analysis showed that the ANI value of genomes of strain 524-17T and P. phocaeensis strain YL31(NZ CP015406) is 73.1% and the dDDH estimation is 21.3%. Based on these results, we concluded that the strain 524-17T represents of new species of the genus Pseudoflavonifractor, and the name Pseudoflavonifractor muris sp. nov. is proposed. Cells are strictly anaerobic, straight rods (4–8 μm long by 0.4–0.6 μm wide) with peritrichous flagella. Circular, whitsh, smooth, entire, lightly raise translucent colonies appear on MGAM agar plate after 5 days of incubation. Growth occurs at temperature range of 30-37 °C and at pH range of 7.0-7.5. The organism uses dextrin, D-fructose, L-fucose, D-galactose, D-galacturonic acid, gentiobiose, D-glucosaminic acid, glucose-6-phosphate, D-melibiose, 3-melthyl-D-glucose and L-Phenylalanine as the carbon source for growth, and weakly metabolizes amygdalin arbutin, D-cellobiose, α-D-glucose, glucose-1-phosphate, α-D-lactose, maltose, maltotriose, D-mannose, α-methyl-D-glucoside, salicin, sucrose, D-trehalose, pyruvic acid, L, methionine, L-valine and L-valine plus L-aspartic acid. The DNA G+C content of the type strain is 58 mol%.
Taxonomy marker
MK287742
Pseudoflavonifractor sp.
Create date: 17-DEC-2018
Length
1246
Topology
linear
Strandedness
double
Genomics
Submitter date: 2019-12-30 14:40:37 Update date: 2019-12-30 14:40:37
Source
Chang Liu, Institute of Microbiology, Chinese Academy of Science