General information
Name
'Roseburia muris'
Nomenclature type
D42-69T;CGMCC 1.31207
Etymology
mu’ris L. gen. n. muris of a mouse
Ecology
Isolation source
isolated from the cecal contents of the 8-week-old male Lepob/ob C57BL/6J mouse.
Morphology
The taxon 99, respresented by strain D42-69T, is phylogenetically closest to Roseburia intestinalis39, with 16S rRNA gene identity of 95%. Phylogenetic tree shows that strain D42-69T clusters with other members of the genus Roseburia, suggesting strain D42-69T is a member of the genus Roseburia. The genome of strain D42-69T was sequenced and the GenBank accession number is RAZG00000000. Genome-based analyses showed that the ANI value of genomes of strain D42-69T and R. intestinalis L1-82 Scfld102(NZ GG692814) is 69.8% and the dDDH estimation is 23.7%. Based on these results, we concluded that the strain D42-69T represents of new species of the genus Roseburia, and the name Roseburia muris sp. nov. is proposed. Cells are strictly anaerobic rods (2–3 μm long by 0.4–0.6 μm wide). Punctiform, yellow or light brown, lightly flat with non-translucent colonies appear on MGAM agar plate after 4 days of incubation. Growth occurs at temperature of 30-37 °C and at pH of 7.0-7.5. The organism can use D-fructose, L-fucose, D-galactose, D-galacturonic acid, gentiobiose, D-glucosaminic acid, glucose-6-phosphate, lactulose, D-mannose, D-melibiose, 3-melthyl-D-glucose, palatinose and L-rhamnose as the carbon source for growth, and weakly metabolizes α-D-glucose, M-inositol, α-D-lactose and pyruvic acid. The DNA G+C content is 42.6 mol%.
Taxonomy marker
MK287766
Roseburia sp.
Create date: 17-DEC-2018
Length
1403
Topology
linear
Strandedness
double
Genomics
Submitter date: 2019-12-30 14:40:37 Update date: 2019-12-30 14:40:37
Source
Chang Liu, Institute of Microbiology, Chinese Academy of Science