General Information
- Experiment ID
- OEX001906
- Experiment Name
- OEX_Peiwei_1909030422
- Experiment Type
- Other
- Description
- Total RNA was extracted from six OVM samples and two normal vascular tissues. OVM samples were collected into TRIzol (GIBCO) immediately after surgical resection. Normal vascular tissues were obtained in the cosmetic eyelid surgery.
- Protocol
- We used GeneChip Human Genome U133A 2.0 Array (Affymetirx) for the microarray analyses as described earlier. Each sample were re-suspended in 1000 μL of Trizol and total RNA was isolated using the MagMAX™-96 for Microarrays Total RNA Isolation Kit (InvitrogenTM Life Technologies, Carlsbad, CA). Hybridization was done in Affymetrix hybridization oven followed by washing and scanning in Fluidic station 450 and Genechip Scanner respectively utilizing GCOS software. Data interpretation and analysis was done through GCOS manager software specifications.
Experiment information
- Attributes
-
library_strategy n/a library_layout n/a library_construction n/a library_selection n/a mate_pair n/a platform Other insertion_size n/a library_name n/a - Project
-
1
Project ID Project name Create Date OEP000463 Orbital Venous malformation 2019-09-03 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES019362 OVM-1 Homo sapiens N/A 2019-09-03 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER033009 OER_Peiwei_1909030430 OVM-1 8 2019-09-03
Author Information
- Create Date
- 2019-09-03
- Last Modified
- 2019-09-03
- Submission
- Peiwei Chai
- Shanghai Ninth people's hospital