General Information
- Experiment ID
- OEX011559
- Experiment Name
- bacteria_AXX1
- Experiment Type
- Genomic
- Protocol
- Nuclei from formaldehyde crosslinked (1%; 10min at room temperature) embryos were isolated and chromatin was fragmented using sonicator (Branson). Lysates were cleared and protein-DNA complexes were isolated using target specific antibodies and Dynabeads. DNA fragments were purified and libraries were prepared by NEBnext DNA Library Prep Kit (NEB, E7370L). Adapter ligated DNA was purified using AMPure XP beads (Beckman Coulter, A63881) and sequenced by illumina HiSeq 2500. NEBnext DNA Library Prep Kit
Experiment information
- Attributes
-
library_strategy AMPLICON library_selection PCR library_layout Paired mate_pair N platform Illumina HiSeq 2500 - Project
-
1
Project ID Project name Create Date OEP001579 urban wetland microbial community 2021-01-13 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES064979 bacteria_AXX1 pond metagenome N/A 2021-01-13 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER098527 bacteria_AXX1 bacteria_AXX1 1 2021-01-13
Author Information
- Create Date
- 2021-01-13
- Last Modified
- 2023-04-14
- Submission
- Binhao Wang
- Sun Yat-sen University