Project IDOEP005300
Project NameFecal metagenomics of mice treated with Bifidobacterium infantis
Experiment TypeMetagenomic
ProtocolFirstly, sixteen male BALB/c mice weighing 14–18 g were purchased from Pengyue Experimental Animal Breeding Co., Ltd. (Jinan, China) and kept under a 12h light/12h dark cycle in standard specific-pathogen-free (SPF) individually vented cages. The mice were given a common diet and free access to water. Mice aged 3 weeks were randomly divided into experimental groups of eight mice, as follows: Control group (gavage with physiological saline (0.9 % NaCl) (150 µL) for 12 days and intraperitoneal injection with physiological saline (0.9 % NaCl) on the 13th day) (Fig.1a); B. infantis group (gavage with 109 CFU/mL B. infantis 15697 (150 µL) for 12 days and intraperitoneal injected physiological saline (0.9 % NaCl) in 13th days) (Fig.1a). After 4 h of intraperitoneal injection, mice were anesthetized and cervical dislocated. Samples (e.g. liver tissue, ileum tissue, ileum chyme, and feces) were taken, and stored in 4 % paraformaldehyde and liquid nitrogen respectively.
OEX027843
Project IDOEP005268
Project NameThe mNGS analysis of infective endocarditis
Experiment TypeGenomic
ProtocolFirst of all, we constructed a protein sequence database tailored to IE used for metaproteomic analyses, derived from matched metagenomes, which were generated from metagenomic next-generation sequencing (mNGS) of ten aliquots pooled and packaged from 158 vegetation samples that matched the ones used for metaproteomics. The metagenomic sequence data were strictly processed through quality control and filtering of host reads. Subsequently, we assembled all the reads of the ten samples together and performed open reading frame (ORF) prediction analysis to obtain the final gene catalogue for subsequent analysis.
OEX027817
Project IDOEP005278
Project NameECS based on NGS in Southern Chinese couples
Experiment TypeGenomic
OEX027808
Project IDOEP005267
Project NameHspa1b
Experiment TypeTranscriptomic
OEX027799
Project IDOEP005260
Project NameKD_BAs
Experiment TypeTranscriptomic
ProtocolTruSeq RNA Sample Preparation kit
OEX027793
Project IDOEP005260
Project NameKD_BAs
Experiment TypeMetabolomic
OEX027796
Project IDOEP005260
Project NameKD_BAs
Experiment TypeMetabolomic
OEX027795
Project IDOEP005245
Project NameNative microbial populations in the alkaline mines in Panxi Area, Southwest China
Experiment TypeGenomic
OEX027776
Project IDOEP005245
Project NameNative microbial populations in the alkaline mines in Panxi Area, Southwest China
Experiment TypeGenomic
OEX027777
Project IDOEP004885
Project NameCross-feeding interactions among the major degrader and rare non-degraders in a bacterial consortium reveal the role of bacterial dark matter in lignin degradation
Experiment TypeGenomic
OEX026786