Project IDOEP005095
Project NameThe transcriptome sequencing of E. coli with AMPs
Experiment TypeGenomic
OEX027350
Project IDOEP005095
Project NameThe transcriptome sequencing of E. coli with AMPs
Experiment TypeGenomic
OEX027374
Project IDOEP005095
Project NameThe transcriptome sequencing of E. coli with AMPs
Experiment TypeGenomic
OEX027363
Project IDOEP005095
Project NameThe transcriptome sequencing of E. coli with AMPs
Experiment TypeGenomic
OEX027376
Project IDOEP005095
Project NameThe transcriptome sequencing of E. coli with AMPs
Experiment TypeGenomic
OEX027364
Project IDOEP005095
Project NameThe transcriptome sequencing of E. coli with AMPs
Experiment TypeGenomic
OEX027353
Project IDOEP005091
Project NameStool 16s rRNA sequencing of mice with thigh infection
Experiment TypeGenomic
OEX027343
Project IDOEP005172
Project NameN/A
Experiment TypeGenomic
Protocoln/a
OEX027665
Project IDOEP005161
Project NameVLS 16S DATA in girls
Experiment TypeTranscriptomic
OEX027664
Project IDOEP004403
Project NameH3K4me3 ChIP-seq of CD4+T cell from SLE patients and HC
Experiment TypeGenomic
ProtocolChIP-seq was performed as previously described. Briefly, 2-5 million CD4+ T cells were lysed in cell lysis buffer for 5 min on ice. Cell were pelleted and resuspened with 200 μl of digestion buffer. Digestion was then performed by adding micrococcal nuclease and incubation for 5 min at 37 °C. The reaction was stopped by EDTA-EGTA buffer . After centrifuge for 15 min at 13000rpm, 1/10 of the supernatant were taken as Input sample, others supernatant was dilute into a CHIP-IP buffer. Chromatin was then incubated overnight at 4 °C with anti-trimethylated H3K4 antibody . Protein G magnetic beads were added for an additional 4 h of incubation. Immunoprecipitates were washed vigorously and DNA was purified with PCR purification kit. 1ng-50ng H3K4me3 ChIP'ed DNA or input DNA was used for ChIP-seq library construction via TruePrep DNA Library Prep Kit V2 for Illumina. After PCR step, the DNA libraries were purified and size selection by 0.5× and 0.25× DNA beads. ChIP-seq libraries were sequenced via paired-end 150 bp reads on an Illumina platform.
OEX024597