General Information
- Project ID
- OEP001507
- Project Name
- Decoding Human Megakaryocyte Development
- Description
- Despite our growing understanding of embryonic immune development, rare early megakaryocytes (MKs) remain relatively understudied. Here we used single-cell RNA sequencing of human MKs from embryonic yolk sac (YS) and fetal liver (FL) to characterize the transcriptome, cellular heterogeneity, and developmental trajectories of early megakaryopoiesis. In the YS and FL, we found heterogeneous MK subpopulations with distinct developmental routes and patterns of gene expression that could reflect early functional specialization. Intriguingly, we identified a subpopulation of CD42b+CD14+ MKs in vivo that exhibit high expression of genes associated with immune responses and can also be derived from human embryonic stem cells (hESCs) in vitro. Furthermore, we identified THBS1 as an early marker for MK-biased embryonic endothelial cells. Overall, we provide important insights and invaluable resources for dissection of the molecular and cellular programs underlying early human megakaryopoiesis.
- Publications
-
Decoding Human Megakaryocyte Development
Wang H, He J, Xu C, et al. Decoding Human Megakaryocyte Development [published online ahead of print, 2020 Dec 9]. Cell Stem Cell. 2020;S1934-5909(20)30544-0.
(PMID: 33340451) (DOI: 10.1016/j.stem.2020.11.006)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX011324 Transcriptomic single cell Transcriptomic single cell 2021-01-10 - Samples
- 26
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES060372 human_YS_MK_1_1 Human Homo sapiens Yolk sac 2021-01-10 OES060373 human_YS_MK_1_2 Human Homo sapiens Yolk sac 2021-01-10 OES060374 human_YS_MK_2_1 Human Homo sapiens Yolk sac 2021-01-10 OES060375 human_YS_MK_2_2 Human Homo sapiens Yolk sac 2021-01-10 OES060376 human_YS_MK_3_1 Human Homo sapiens Yolk sac 2021-01-10 OES060377 human_YS_MK_3_2 Human Homo sapiens Yolk sac 2021-01-10 OES060378 FKDL202577818-1a_L6_1 Human Homo sapiens Fetal liver 2021-01-10 OES060379 FKDL202577818-1a_L6_2 Human Homo sapiens Fetal liver 2021-01-10 OES060380 FKDL202577818-1a_L7_1 Human Homo sapiens Fetal liver 2021-01-10 OES060381 FKDL202577818-1a_L7_2 Human Homo sapiens Fetal liver 2021-01-10 OES060382 FKDL202577819-1a_L6_1 Human Homo sapiens Fetal liver 2021-01-10 OES060383 FKDL202577819-1a_L6_2 Human Homo sapiens Fetal liver 2021-01-10 OES060384 FKDL202577819-1a_L7_1 Human Homo sapiens Fetal liver 2021-01-10 OES060385 FKDL202577819-1a_L7_2 Human Homo sapiens Fetal liver 2021-01-10 OES060386 FKDL202577820-1a_L7_1 Human Homo sapiens Fetal liver 2021-01-10 OES060387 FKDL202577820-1a_L7_2 Human Homo sapiens Fetal liver 2021-01-10 OES060388 FKDL202577820-1a_L8_1 Human Homo sapiens Fetal liver 2021-01-10 OES060389 FKDL202577820-1a_L8_2 Human Homo sapiens Fetal liver 2021-01-10 OES060390 FKDL202577821-1a_L6_1 Human Homo sapiens Fetal liver 2021-01-10 OES060391 FKDL202577821-1a_L6_2 Human Homo sapiens Fetal liver 2021-01-10 OES060392 FKDL202577821-1a_L7_1 Human Homo sapiens Fetal liver 2021-01-10 OES060393 FKDL202577821-1a_L7_2 Human Homo sapiens Fetal liver 2021-01-10 OES060394 FKDL202577822-1a_L6_1 Human Homo sapiens Fetal liver 2021-01-10 OES060395 FKDL202577822-1a_L6_2 Human Homo sapiens Fetal liver 2021-01-10 OES060396 FKDL202577822-1a_L7_1 Human Homo sapiens Fetal liver 2021-01-10 OES060397 FKDL202577822-1a_L7_2 Human Homo sapiens Fetal liver 2021-01-10 - Runs
-
26
Run ID Run Name Experiment Sample Data Num Create Date OER094670 human_YS_MK_1_1 OEX011324 OES060372 1 2021-01-10 OER094671 human_YS_MK_1_2 OEX011324 OES060373 1 2021-01-10 OER094672 human_YS_MK_2_1 OEX011324 OES060374 1 2021-01-10 OER094673 human_YS_MK_2_2 OEX011324 OES060375 1 2021-01-10 OER094674 human_YS_MK_3_1 OEX011324 OES060376 1 2021-01-10 OER094675 human_YS_MK_3_2 OEX011324 OES060377 1 2021-01-10 OER094676 FKDL202577818-1a_L6_1 OEX011324 OES060378 1 2021-01-10 OER094677 FKDL202577818-1a_L6_2 OEX011324 OES060379 1 2021-01-10 OER094678 FKDL202577818-1a_L7_1 OEX011324 OES060380 1 2021-01-10 OER094679 FKDL202577818-1a_L7_2 OEX011324 OES060381 1 2021-01-10 OER094680 FKDL202577819-1a_L6_1 OEX011324 OES060382 1 2021-01-10 OER094681 FKDL202577819-1a_L6_2 OEX011324 OES060383 1 2021-01-10 OER094682 FKDL202577819-1a_L7_1 OEX011324 OES060384 1 2021-01-10 OER094683 FKDL202577819-1a_L7_2 OEX011324 OES060385 1 2021-01-10 OER094684 FKDL202577820-1a_L7_1 OEX011324 OES060386 1 2021-01-10 OER094685 FKDL202577820-1a_L7_2 OEX011324 OES060387 1 2021-01-10 OER094686 FKDL202577820-1a_L8_1 OEX011324 OES060388 1 2021-01-10 OER094687 FKDL202577820-1a_L8_2 OEX011324 OES060389 1 2021-01-10 OER094688 FKDL202577821-1a_L6_1 OEX011324 OES060390 1 2021-01-10 OER094689 FKDL202577821-1a_L6_2 OEX011324 OES060391 1 2021-01-10 OER094690 FKDL202577821-1a_L7_1 OEX011324 OES060392 1 2021-01-10 OER094691 FKDL202577821-1a_L7_2 OEX011324 OES060393 1 2021-01-10 OER094692 FKDL202577822-1a_L6_1 OEX011324 OES060394 1 2021-01-10 OER094693 FKDL202577822-1a_L6_2 OEX011324 OES060395 1 2021-01-10 OER094694 FKDL202577822-1a_L7_1 OEX011324 OES060396 1 2021-01-10 OER094695 FKDL202577822-1a_L7_2 OEX011324 OES060397 1 2021-01-10
Author Information
- Create Date
- 2021-01-10
- Last Modified
- 2021-04-06
- Submission
- Changlu Xu
- Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College