General Information
- Project ID
- OEP004908
- Project Name
- ARID2 mitigates hepatic steatosis via promoting the ubiquitination of JAK2 (house mouse)
- Description
- Non-alcoholic fatty liver disease (NAFLD) has become a growing public health problem. However, the complicated pathogenesis of NAFLD contributes to the deficiency of effective clinical treatment. Here, we elucidated that liver-specific loss of Arid2 induced hepatic steatosis and this progression could be exacerbated by HFD. Mechanistic study revealed that ARID2 repressed JAK2-STAT5-PPARγ signaling pathway by promoting the ubiquitination of JAK2, which was mediated by NEDD4L, a novel E3 ligase for JAK2. ChIP assay revealed that ARID2 recruited CARM1 to increase H3R17me2 level at NEDD4L promoter and activated the transcription of NEDD4L. Moreover, inhibition of Jak2 by Fedratinib in liver-specific Arid2 knockout mice alleviated HFD-induced hepatic steatosis. Downregulation of ARID2 and the reverse correlation between ARID2 and JAK2 were also observed in clinical samples. Therefore, our study has revealed an important role of ARID2 in the development of NAFLD and provides a potential therapeutic strategy for NAFLD. Overall design: Comparative gene expression profiling analysis of RNA-seq data for livers of Arid2 WT and LKO mice.
- Publications
-
ARID2 mitigates hepatic steatosis via promoting the ubiquitination of JAK2
Cao HJ, Jiang H, Ding K, et al. ARID2 mitigates hepatic steatosis via promoting the ubiquitination of JAK2. Cell Death Differ. 2023;30(2):383-396. doi:10.1038/s41418-022-01090-0
(PMID: 36396719) (DOI: 10.1038/s41418-022-01090-0) - Related Links
- https://www.nature.com/articles/s41418-022-01090-0
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX026987 transcriptomic liver experiment Transcriptomic 2023-12-27 - Samples
- 6
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES345420 sample 059 Animalia Mus musculus liver 2023-12-27 OES345421 sample 060 Animalia Mus musculus liver 2023-12-27 OES345422 sample 061 Animalia Mus musculus liver 2023-12-27 OES345423 sample 062 Animalia Mus musculus liver 2023-12-27 OES345424 sample 063 Animalia Mus musculus liver 2023-12-27 OES345425 sample 064 Animalia Mus musculus liver 2023-12-27 - Runs
-
6
Run ID Run Name Experiment Sample Data Num Create Date OER452448 SRR21982059 OEX026987 OES345420 2 2023-12-27 OER452449 SRR21982060 OEX026987 OES345421 2 2023-12-27 OER452450 SRR21982061 OEX026987 OES345422 2 2023-12-27 OER452451 SRR21982062 OEX026987 OES345423 2 2023-12-27 OER452452 SRR21982063 OEX026987 OES345424 2 2023-12-27 OER452453 SRR21982064 OEX026987 OES345425 2 2023-12-27
Author Information
- Create Date
- 2023-12-27
- Last Modified
- 2024-02-05
- Submission
- Jingjing Li
- Shanghai Institute of Nutrition and Health, CAS