General Information
- Project ID
- OEP005241
- Project Name
- Altered nucleocytoplasmic export of adenosine (A)-rich circRNAs by PABPC1 contributes to neuronal function
- Description
- Circular RNAs (circRNAs) are upregulated during neurogenesis. Where and how circRNAs are localized, and what roles they play during this process have remained elusive. By comparing the nuclear and cytoplasmic circRNAs between H9 cells and H9-derived forebrain neurons (FB), we found a subset of adenosine (A)-rich circRNAs are restricted in H9 nuclei but exported to cytosols upon forebrain neuron differentiation. This differentiation-coupled circRNA subcellular relocation is modulated by the poly(A)-binding protein PABPC1. In the nucleus, newly produced (A)-rich circRNAs are bound by PABPC1 and the nuclear basket protein TPR to prevent their nucleocytoplasmic export. Modulation of (A)-rich motifs in circRNAs remarkably alters their subcellular localization. Enforced (A)-rich circRNAs in cytosols result in mRNA translation suppression. Furthermore, decreased nuclear PABPC1 upon neuronal differentiation enables the export of (A)-rich circRNAs including circRTN4(2,3), which is required for neurite outgrowth. These findings uncover subcellular localization features of circRNAs, linking their processing and function during neurogenesis.
Project information
- Experiments
- 2
- Samples
- 12
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES372347 FB total cell ribo– RNA-seq replicate1 Cell line Homo sapiens cell line 2024-04-27 OES372348 FB total cell ribo– RNA-seq replicate2 Cell line Homo sapiens cell line 2024-04-27 OES372349 FB cytoplasm ribo– RNA-seq replicate1 Cell line Homo sapiens cell line 2024-04-27 OES372350 FB cytoplasm ribo– RNA-seq replicate2 Cell line Homo sapiens cell line 2024-04-27 OES372351 FB nuclear ribo– RNA-seq replicate1 Cell line Homo sapiens cell line 2024-04-27 OES372352 FB nuclear ribo– RNA-seq replicate2 Cell line Homo sapiens cell line 2024-04-27 OES372353 FB scramble ribo– RNA-seq replicate1 Cell line Homo sapiens cell line 2024-04-28 OES372354 FB scramble ribo– RNA-seq replicate2 Cell line Homo sapiens cell line 2024-04-28 OES372355 FB circRTN4(2,3) shRNA1 KD ribo– RNA-seq replicate1 Cell line Homo sapiens cell line 2024-04-28 OES372356 FB circRTN4(2,3) shRNA1 KD ribo– RNA-seq replicate2 Cell line Homo sapiens cell line 2024-04-28 OES372357 FB circRTN4(2,3) shRNA2 KD ribo– RNA-seq replicate1 Cell line Homo sapiens cell line 2024-04-28 OES372358 FB circRTN4(2,3) shRNA2 KD ribo– RNA-seq replicate2 Cell line Homo sapiens cell line 2024-04-28 - Runs
-
12
Run ID Run Name Experiment Sample Data Num Create Date OER470212 FB_total_RNA_rep1 OEX027770 OES372347 2 2024-04-28 OER470214 FB_cytoplasm_rep1 OEX027770 OES372349 2 2024-04-28 OER470215 FB_nuclear_rep2 OEX027770 OES372352 2 2024-04-28 OER470216 FB_cytoplasm_rep2 OEX027770 OES372350 2 2024-04-28 OER470217 FB_nuclear_rep1 OEX027770 OES372351 2 2024-04-28 OER470218 FB_total_RNA_rep2 OEX027770 OES372348 2 2024-04-28 OER470219 FB_scramble_rep1 OEX027771 OES372353 2 2024-04-28 OER470220 FB_scramble_rep2 OEX027771 OES372354 2 2024-04-28 OER470221 FB_circRTN4_shRNA1_rep1 OEX027771 OES372355 2 2024-04-28 OER470222 FB_circRTN4_shRNA1_rep2 OEX027771 OES372356 2 2024-04-28 OER470223 FB_circRTN4_shRNA2_rep1 OEX027771 OES372357 2 2024-04-28 OER470224 FB_circRTN4_shRNA2_rep2 OEX027771 OES372358 2 2024-04-28
Author Information
- Create Date
- 2024-04-27
- Last Modified
- 2024-04-30
- Submission
- Li Yang
- Fudan University