General Information
- Sample ID
- OES074566
- Sample Name
- SDHB-methyl samples
- Description
- The villi of 13 normal pregnant women and 7 RSA patients were collected. After genome extraction, the SDHB promoter methylation was determined by MethylTarget sequencing (Genesky Biotechnologies Inc., Shanghai, China), using next-generation sequencing-based multiple targeted CpG methylation analysis.
- Subject type
- Human
Sample information
- Organism
- Homo sapiens
- Tissue
- villi
- Protocol
- Genomic DNA was extracted from frozen samples using Genomic Tip-500 columns (Qiangen, Valencia, CA, USA) and from bisulfite-converted samples using an EZ DNA Methylation™-GOLD Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions.
- ATTRIBUTES
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biomaterial_provider Shanghai First Maternity and Infant Hospital - Projects
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1
Project ID Project name Submitter Date OEP001858 SDHB Promoter MethylTarget Sequencing Xu, Sha 2021-03-10 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX012490 OEX_Sha_2103101650 Genomic In order to quantify the methylation of SDHB promoter in different pregnancy period of health control or RSA patients, DNA methylation at specific CpG sites was determined by MethylTarget sequencing (Genesky Biotechnologies Inc., Shanghai, China), using next-generation sequencing-based multiple targeted CpG methylation analysis. Primer design and validation were performed using Methylation Primer software on bisulfate-converted DNA. Primer sets were designed flanking each targeted CpG site in a 100–300 nucleotide region. Genomic DNA was extracted from frozen samples using Genomic Tip-500 columns (Qiangen, Valencia, CA, USA) and from bisulfite-converted samples using an EZ DNA Methylation™-GOLD Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions. After PCR amplification (HotStarTaq polymerase kit, TAKARA, Tokyo, Japan) and library construction, samples were sequenced (Illumina HiSeq Benchtop Sequencer, CA, USA) by paired-end sequencing according to the manufacturer’s guidelines. 2021-03-10 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER132880 OER_Sha_2103101646 OEP001858 OEX012490 OES074566 1 2021-03-10
Author Information
- Create Date
- 2021-03-10
- Last Modified
- 2021-03-10
- Submission
- Sha Xu,Fudan University