OEX014298
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Genomic |
Genomic |
Tumor sections were reviewed by a pathologist to ensure tumor sections with > 70% tumors and < 10% necrosis. High-quality genomic DNA was extracted by using the GeneRead DNA FFPE Kit (180134, QIAGEN, Hilden, GER) according to the manufacturer’s instructions. Germline DNA (gDNA) was extracted from white blood cells using the Blood Genomic DNA Mini Kit (CW2087, Cwbiotech, Beijing, China). WES was used to determine the mutational landscape of TFE3-tRCC. Exome capture was performed using the Agilent SureSelect Human All ExonV5 kit (Technologies, CA, USA) according to the manufacturer’s instructions. This was followed by paired-end sequencing using Illumina Novaseq6000 sequencer (Illumine Inc, CA, USA). Mean clean sequence data obtained was 38.30 Gb for tumor samples and 14.95 Gb for normal tissue/blood samples. |
2021-07-27 |