General Information
- Sample ID
- OES359817
- Sample Name
- NFs#1
- Description
- Human PCa tissues and their corresponding normal prostate tissues.
- Subject type
- Human
Sample information
- Organism
- Homo sapiens
- Tissue
- prostate
- Protocol
- the tumours and non-tumour tissues underwent mincing and dissociation in RPMI-1640 supplemented with 0.5% collagenase Type I for a duration of 2 h at 37°C. Subsequently, they were cultured in a humidified atmosphere at 37°C with 5% CO2 using RPMI-1640 supplemented with 10% FBS until the fibroblasts adhered to the culture dish.
- ATTRIBUTES
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gender male clinical_treatment surgery - Projects
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1
Project ID Project name Submitter Date OEP005133 Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis Xiong, Zhi 2024-03-15 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX027424 Transcriptome Transcriptomic The TRIzol method was used for the RNA extraction from the prostate cancer tissues. Novogene (Beijing, China) was used to construct the cDNA library. An Illumina NovaSeq 6000 platform (Illumina, Novogene) was used for RNA-seq. RNA-seq data were analyzed using the R package (DESeq). 2024-03-15 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER460888 OER_Zhi_2403151955 OEP005133 OEX027424 OES359817 2 2024-03-15
Author Information
- Create Date
- 2024-03-15
- Last Modified
- 2024-03-15
- Submission
- Zhi Xiong,Sun Yat-sen University