Editable site gRNA design for each BE
Instructions:
1. The input sequence only include A,T,C,G
2. "Or(Union)" means selection for sites that at least one of the chosen BEs can target;"And(intersection)" means selection for sites that all of the chosen BEs can target.
3. For Cas9-based BEs, the position of editing window is counted distal from PAM:
N1N2N3N4N5N6N7N8N9N10N11N12N13N14N15N16N17N18N19N20NGG
For dCpf1(Cas12a)-based BEs,the position of editing window is conted proximal from PAM:
TTTVN1N2N3N4N5N6N7N8N9N10N11N12N13N14N15N16N17N18N19N20N21N22N23
4. Generally,the spacer sequence length of nSpCas9-based BEs is 20; the spacer sequence length of dCpf1 is 23; the spacer length of nSaCas9-based BEs is 21.
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