BEable-GPS: Base Editable prediction of Global Pathogenic-related SNVs

Editable site gRNA design for each BE

Input sequence (min:50nt, max:5000nt, only A,T,C,G are allowed)
- (Optional,default:1-length of the sequence)

    BECasAPOBECUGIPAMEditing windowReference
    BE3nSpCas9rA11NGG4-8Komor et.al,2016
    BE4maxnSpCas9rA12NGG4-8Koblan et.al,2018
    YE1-BE3nSpCas9rA1W90Y/R126E1NGG5-7Kim et.al.,2017
    YEE-BE3nSpCas9rA1W90Y/R126E/R132E1NGG5-6Kim et.al.,2017
    VQR-BE3nSpCas9(VQR)rA11NGAN4-11Kim et.al.,2017
    VRER-BE3nSpCas9(VRER)rA11NGCG3-10Kim et.al.,2017
    SaBE3nSaCas9rA11NNGRRT3-12Kim et.al.,2017
    Sa(KKH)BE3nSaCas9(KKH)rA11NNNRRT3-12Kim et.al.,2017
    eBE-S3nSpCas9rA11+3XfreeNGG4-8Wang et.al.,2017
    dCpf1-eBEdLbfCas12arA11+3XfreeTTTV8-13Li et.al.,2018
    dCpf1-eBE-YEdLbfCas12arA1W90Y/R126E1+3XfreeTTTV10-12Li et.al.,2018
    Target-AIDnSpCas9CDA11NGG2-4Nishida et.al.,2016
    Target-AID-NGnSpCas9(NG)CDA11NG2-4Nishimasu et.al.,2018
    xBE3xSpCas9rA11NG4-8Hu et.al.,2018
    hA3A-eBEnSpCas9hA3A1+3XfreeNGG2-13Wang et.al.,2018
    hA3A-eBE-Y130FnSpCas9hA3AY130F1+3XfreeNGG3-8Wang et.al.,2018
    hA3A-eBE-Y132DnSpCas9hA3AY132D1+3XfreeNGG3-7Wang et.al.,2018
    eA3A-BE3nSpCas9hA3AN37G1NGG4-8(TC)Gehrke et.al.,2018
    BE3-R33AnSpCas9rA1R33A1NGG5-7Grunewald et.al.,2019
    BE3-R33A/K34AnSpCas9rA1R33A/K34A1NGG5-6(TC)Grunewald et.al.,2019
  • PAM:
  • PAM type:3'of spacer 5'of spacer
  • Editing window:- (The position of the spacer)
  • Spacer length:
Instructions:

1. The input sequence only include A,T,C,G

2. "Or(Union)" means selection for sites that at least one of the chosen BEs can target;"And(intersection)" means selection for sites that all of the chosen BEs can target.

3. For Cas9-based BEs, the position of editing window is counted distal from PAM:

N1N2N3N4N5N6N7N8N9N10N11N12N13N14N15N16N17N18N19N20NGG

For dCpf1(Cas12a)-based BEs,the position of editing window is conted proximal from PAM:

TTTVN1N2N3N4N5N6N7N8N9N10N11N12N13N14N15N16N17N18N19N20N21N22N23

4. Generally,the spacer sequence length of nSpCas9-based BEs is 20; the spacer sequence length of dCpf1 is 23; the spacer length of nSaCas9-based BEs is 21.


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