General Information
- Analysis ID
- OEZ014525
- Analysis Name
- Processed_CUT&Tag-seq data
- Description
- To process raw data, reads containing adapter or ploy-N were removed to generate clean reads. Further filtration according to the calculated Q20, Q30 and GC content resulted in high-quality clean reads, which were then aligned to reference human genome GRCh38 using the BWA program (version 0.7.15). The MACS2 package(version 2.2.9.1)was used for peak-calling from the BAM files with q-value < 0.05. HOMER (version 4.11) was applied to the generated peak file and the genome fasta for motif analysis. Peaks were annotated using the annotatePeaks.pl function. For defining differentially accessible peaks, peak files of each sample were first merged using BEDTools (version 2.28.0). The counts of the reads over the bed were then determined for each sample using the multicov function from BEDTools. Finally, differentially accessible peaks were assessed using DESeq2 (version v1.42.0).
Analysis Information
- Analysis Type
- Other
- Pipeline
Target
Analysis Data
Submitter Information
- Create Date
- 2023-12-18
- Last Modified
- 2023-12-18
- Submission
- Yifei Cheng