General Information
- Experiment ID
- OEX012497
- Experiment Name
- OEX_Sha_2103111031
- Experiment Type
- Genomic
- Description
- DNA methylation analysis of health control or RSA patients
- Protocol
- In order to quantify the methylation of SDHB promoter in different pregnancy period of health control or RSA patients, DNA methylation at specific CpG sites was determined by MethylTarget sequencing (Genesky Biotechnologies Inc., Shanghai, China), using next-generation sequencing-based multiple targeted CpG methylation analysis. Primer design and validation were performed using Methylation Primer software on bisulfate-converted DNA. Primer sets were designed flanking each targeted CpG site in a 100–300 nucleotide region. Genomic DNA was extracted from frozen samples using Genomic Tip-500 columns (Qiangen, Valencia, CA, USA) and from bisulfite-converted samples using an EZ DNA Methylation™-GOLD Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions. After PCR amplification (HotStarTaq polymerase kit, TAKARA, Tokyo, Japan) and library construction, samples were sequenced (Illumina HiSeq Benchtop Sequencer, CA, USA) by paired-end sequencing according to the manufacturer’s guidelines.
Experiment information
- Attributes
-
library_layout Paired library_selection PCR library_strategy Bisulfite-Seq platform Illumina HiSeq 4000 - Project
-
1
Project ID Project name Create Date OEP001862 SDHB Promoter MethylTarget Sequencing 2021-03-11 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES074823 SDHB-methyl samples Homo sapiens Villi 2021-03-11 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER132978 OER_Sha_2103111041 SDHB-methyl samples 1 2021-03-11
Author Information
- Create Date
- 2021-03-11
- Last Modified
- 2021-06-10
- Submission
- Sha Xu
- Fudan University