General Information
- Project ID
- OEP001862
- Project Name
- SDHB Promoter MethylTarget Sequencing
- Description
- In order to quantify the methylation of SDHB promoter in different pregnancy period of health control or RSA patients, DNA methylation at specific CpG sites was determined by MethylTarget sequencing (Genesky Biotechnologies Inc., Shanghai, China), using next-generation sequencing-based multiple targeted CpG methylation analysis. Primer design and validation were performed using Methylation Primer software on bisulfate-converted DNA. Primer sets were designed flanking each targeted CpG site in a 100–300 nucleotide region. Genomic DNA was extracted from frozen samples using Genomic Tip-500 columns (Qiangen, Valencia, CA, USA) and from bisulfite-converted samples using an EZ DNA Methylation™-GOLD Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions. After PCR amplification (HotStarTaq polymerase kit, TAKARA, Tokyo, Japan) and library construction, samples were sequenced (Illumina HiSeq Benchtop Sequencer, CA, USA) by paired-end sequencing according to the manufacturer’s guidelines.
- Publications
-
Low embryonic villous succinate accumulation associates with recurrent spontaneous abortion risk
Wang XH, Xu S, Zhou XY, et al. Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Nat Commun. 2021;12(1):3428. Published 2021 Jun 8.
(PMID: 34103526) (DOI: 10.1038/s41467-021-23827-0)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX012497 OEX_Sha_2103111031 Genomic 2021-03-11 - Samples
- 1
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES074823 SDHB-methyl samples Human Homo sapiens Villi 2021-03-11 - Runs
-
1
Run ID Run Name Experiment Sample Data Num Create Date OER132978 OER_Sha_2103111041 OEX012497 OES074823 1 2021-03-11
Author Information
- Create Date
- 2021-03-11
- Last Modified
- 2021-03-11
- Submission
- Sha Xu
- Fudan University