General Information
Sample information
- Organism
- Homo sapiens
- Tissue
- decidua tissue
- Subject id
- n/a
- Batch
- n/a
- Storage type
- n/a
- Storage temperature
- n/a
- Storage location
- n/a
- Storage duration
- n/a
- Protocol
- On arrival, freshly human decidual tissues were transferred rapidly to a 10-cm dish and washed twice with 1x cold PBS(Gibco). Each tissue was trimmed into 1-mm3 pieces and digested for 45 min each at 37℃ with medium containing 1 mg/mL collagenase IV (Gibcol, 17104019) and 1 U/mL dispase II (Gibcol, 17105041). The cell suspensions were filtered through a 40-μm cell-strainer nylon mesh(BD) and were collected by centrifuging at 700 × g for 10 min. Dissociated cells were washed with MACS buffer (PBS containing 1% FBS, 0.5% EDTA, and 0.05% gentamycin).
- Extracted mol type
- n/a
- Extraction reagent
- n/a
- Treatment
- n/a
- Sample loc
- n/a
- ATTRIBUTES
-
gender female health_status Health - Projects
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1
Project ID Project name Submitter Date OEP002901 The recurrent spontaneous abortion (RSA) patients' abnormal decidual microenvironment Chen, Zechuan 2021-11-15 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX014793 OEP002901_HD_scRNAseq Transcriptomic single cell On arrival, freshly human decidual tissues were transferred rapidly to a 10-cm dish and washed twice with 1x cold PBS(Gibco). Each tissue was trimmed into 1-mm3 pieces and digested for 45 min each at 37℃ with medium containing 1 mg/mL collagenase IV (Gibcol, 17104019) and 1 U/mL dispase II (Gibcol, 17105041). The cell suspensions were filtered through a 40-μm cell-strainer nylon mesh(BD) and were collected by centrifuging at 700 × g for 10 min. Dissociated cells were washed with MACS buffer (PBS containing 1% FBS, 0.5% EDTA, and 0.05% gentamycin). 2021-11-15 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER234682 1_HD OEP002901 OEX014793 OES127986 15 2021-11-15
Author Information
- Create Date
- 2021-11-15
- Last Modified
- 2022-01-18
- Submission
- Zechuan Chen,Chinese Academy of Sciences