General Information
- Sample ID
- OES191026
- Sample Name
- IP-MS of MRG15
- Description
- Immunoprecipitation-mass spectrometry (IP-MS) of MRG15 in HEK 293T cells
- Subject type
- Human
Sample information
- Organism
- Homo sapiens
- Tissue
- cell line
- Protocol
- FLAG-MRG15 was overexpressed in HEK 293T cells, with the cell pellets collected after 48 hours and lysed with RIPA buffer (Millipore, 20188). FLAG-MRG15 was then immunoprecipitated with anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, M8823) at 4 °C overnight. The M2 beads were washed with RIPA buffer (Millipore, 20188) for three times, and binding proteins were eluted with 3× FLAG peptide (Sigma-Aldrich, F4799). The eluate from M2 beads was subjected to mass spectrometry analysis.
- Projects
-
1
Project ID Project name Submitter Date OEP003465 IP-MS of FLAG-MRG15 in HEK 293T Tian, Cheng 2022-06-21 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX018169 OEX_Cheng_2206211625 Proteomic FLAG-MRG15 was overexpressed in HEK 293T cells, with the cell pellets collected after 48 hours and lysed with RIPA buffer (Millipore, 20188). FLAG-MRG15 was then immunoprecipitated with anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, M8823) at 4 °C overnight. The M2 beads were washed with RIPA buffer (Millipore, 20188) for three times, and binding proteins were eluted with 3× FLAG peptide (Sigma-Aldrich, F4799). The eluate from M2 beads was subjected to mass spectrometry analysis. 2022-06-21 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER257727 OER_Cheng_2206211640 OEP003465 OEX018169 OES191026 1 2022-06-21
Author Information
- Create Date
- 2022-06-21
- Last Modified
- 2022-06-21
- Submission
- Cheng Tian,Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences