General Information
- Sample ID
- OES298907
- Sample Name
- Dive119_DOES_R
- Description
- The collected sediments were preserved in 70 MPa high pressure-retaining vessels on shipboard and stored at 4 °C until used for subsequent experiments. Sampling of the sediment is performed when switching next incubation pressure during continuous incubation for metatranscriptomic sequencing.
- Subject type
- Environment non-host
Sample information
- Organism
- marine sediment metagenome
- Protocol
- The purified RNA is quantified using a Qubit 4.0 Fluorometer, and is converted to cDNA, then the metatranscriptome library is constructed by using Illumina TruSeq Stranded mRNA LT Sample Prep Kit for subsequent sequencing.
- ATTRIBUTES
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env_biome marine biome (ENVO:00000447) lat_lon 142.2516°E, 11.6639°N depth 6002 m biome root:Environmental:Aquatic:Marine:Sediment env_package sediment - Projects
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1
Project ID Project name Submitter Date OEP004045 Metatranscriptomics during DOES incubation under different HHP Zhang, Yu 2023-03-15 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX022922 Metatranscriptomics sequencing of DOES incubation samples Metatranscriptomic To ensure DNA removal, the RNA extracts are treated with TURBO DNase (Invitrogen, Waltham, MA, USA) as directed by the manufacturer. 2023-03-15 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER383466 Metatranscriptomic during DOES incubation OEP004045 OEX022922 OES298907 0 2023-03-15
Author Information
- Create Date
- 2023-03-15
- Submission
- Yu Zhang,