General Information
- Experiment ID
- OEX022922
- Experiment Name
- Metatranscriptomics sequencing of DOES incubation samples
- Experiment Type
- Metatranscriptomic
- Description
- The extraction of RNA from sediment samples are carried out using the RNeasy® PowerSoil® Total RNA Kit (Qiagen, Germany) according to the manufacturer’s instructions for metatranscriptomics sequencing.
- Protocol
- To ensure DNA removal, the RNA extracts are treated with TURBO DNase (Invitrogen, Waltham, MA, USA) as directed by the manufacturer.
Experiment information
- Attributes
-
library_layout Paired library_selection RANDOM library_strategy RNA-Seq platform Illumina NovaSeq 6000 - Project
-
1
Project ID Project name Create Date OEP004045 Metatranscriptomics during DOES incubation under different HHP 2023-03-15 - Samples
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7
Sample ID Sample Name Organism Tissue Create Date OES298907 Dive119_DOES_R marine sediment metagenome N/A 2023-03-15 OES298171 DY37-II-Dive119-preserved in 70 MPa HP vessel marine sediment metagenome N/A 2023-03-13 OES338108 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 90 MPa marine sediment metagenome N/A 2023-12-05 OES338107 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 70 MPa marine sediment metagenome N/A 2023-12-05 OES338106 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 40 MPa marine sediment metagenome N/A 2023-12-05 OES338109 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 115 MPa marine sediment metagenome N/A 2023-12-05 OES338105 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 0.1 MPa marine sediment metagenome N/A 2023-12-05 - Runs
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7
Run ID Run Name Sample Data Num Create Date OER383466 Metatranscriptomic during DOES incubation Dive119_DOES_R 0 2023-03-15 OER444816 DOES_MT_initial DY37-II-Dive119-preserved in 70 MPa HP vessel 2 2023-12-05 OER444817 DOES_MT_90 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 90 MPa 2 2023-12-05 OER444818 DOES_MT_70 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 70 MPa 2 2023-12-05 OER444819 DOES_MT_40 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 40 MPa 2 2023-12-05 OER444820 DOES_MT_115 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 115 MPa 2 2023-12-05 OER444821 DOES_MT_0.1 DY37-II-Dive119-preserved in 70 MPa HP vessel incubated under 0.1 MPa 2 2023-12-05
Author Information
- Create Date
- 2023-03-15
- Last Modified
- 2024-03-03
- Submission
- Yu Zhang