OENC20 Catalytic biopart Map View
experimental evidence
Name
Organism
Glycine max
Optimum pH
  • 8.0
Optimum temperature(ºC)
  • 30.0
Chassis
  • Escherichia coli BL21(DE3)
Submitter
Xiaodong Li
CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Science, Fenglin Rd 300, Shanghai 200153, China
Submission date
2020-01-01
Last update
2020-01-01
Catalytic functions

1) Daidzein + S-adenosylmethionine => Formononetin + s-adenosylhomocysteine

Daidzein
PUBCHEM: 5281708
Formula: C15H10O4
+
S-adenosylmethionine
PUBCHEM: 34755
Formula: C15H22N6O5S
=>
Formononetin
PUBCHEM : 5280378
Formula: C16H12O4
+
s-adenosylhomocysteine
PUBCHEM : 439155
Formula: C14H20N6O5S

Bioparts catalyze this reaction

Biopart ID Organisms pH temperature(ºC) km

2) Glycetein + S-adenosylmethionine => 4'-O-Methylglycitein + s-adenosylhomocysteine

Glycetein
PUBCHEM: 5317750
Formula: C16H12O5
+
S-adenosylmethionine
PUBCHEM: 34755
Formula: C15H22N6O5S
=>
4'-O-Methylglycitein
Formula: C17H14O5
+
s-adenosylhomocysteine
PUBCHEM : 439155
Formula: C14H20N6O5S

Bioparts catalyze this reaction

Biopart ID Organisms pH temperature(ºC) km
Qualitative and quantitative parameters:
-

Temperature

Temperature optimum(ºC)
  • 30.0
-

pH

pH optimum
  • 8.0
-
Organism

Taxonomy

Taxonomy ID
3847
Organism
Glycine max
Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Glycine subgen. Soja;
-
Sequence and structure

Protein sequence

Protein ID
OENC20.p
Xref
-

Sequence

TESTMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSAR静态页面数据

Nucleotide sequences (Optimized nucleotide sequences according to different chassises)

NT seq ID
OENC20.n.1
Gene name
-

Sequence

ASGSDGLJSLKJGOJSIDJIOJG静态数据

Biopart expression

The gene of biopart was ligated into the expression vector pGEX4T-1 and expressed in E. coli BL21(DE3) under IPTG induction.