OENC40436
Catalytic biopart
Map View
experimental evidence
Organism
Chassis
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Escherichia coli strain XL 1-Blue , Escherichia coli cells
Catalytic functions
External Links
EC number
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5.4.3.5 D-ornithine 4,5-aminomutase.
Bioparts catalyze this reaction
| Biopart ID | Organisms | pH | temperature(ºC) | km |
|---|
Qualitative and quantitative parameters:
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Temperature
pH
Organism
Taxonomy
Taxonomy ID
Organism
Lineage
cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Clostridiales; Peptostreptococcaceae; Acetoanaerobium; Acetoanaerobium sticklandii;
Sequence and structure
Protein sequence
Protein ID
Xref
Sequence
TESTMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSAR静态页面数据
Protein family
Protein structure
Nucleotide sequences (Optimized nucleotide sequences according to different chassises)
NT seq ID
Sequence
ASGSDGLJSLKJGOJSIDJIOJG静态数据
References
- R Somack; R N Costilow; . 1973: Purification and properties of a pyridoxal phosphate and coenzyme B 12 dependent D- -ornithine 5,4-aminomutase. Biochemistry, 12 2597-604.
- H P Chen; S H Wu; Y L Lin; C M Chen; S S Tsay; . 2001: Cloning, sequencing, heterologous expression, purification, and characterization of adenosylcobalamin-dependent D-ornithine aminomutase from Clostridium sticklandii. The Journal of Biological Chemistry, 276 44744-50.
- Cheng-Hsing Tseng; Cheng-His Yang; Heng-Ju Lin; Chunhung Wu; Hao-Ping Chen; . 2007: The S subunit of D-ornithine aminomutase from Clostridium sticklandii is responsible for the allosteric regulation in D-alpha-lysine aminomutase. FEMS Microbiology Letters, 274 148-53.
- Kirsten R Wolthers; Stephen E J Rigby; Nigel S Scrutton; . 2008: Mechanism of radical-based catalysis in the reaction catalyzed by adenosylcobalamin-dependent ornithine 4,5-aminomutase. The Journal of Biological Chemistry, 283 34615-25.
- Nuria Fonknechten; Alain Perret; Nadia Perchat; Sabine Tricot; Christophe Lechaplais; David Vallenet; Carine Vergne; Anne Zaparucha; Denis Le Paslier; Jean Weissenbach; Marcel Salanoubat; . 2009: A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine. Journal of Bacteriology, 191 3162-7.
- Kirsten R Wolthers; Colin Levy; Nigel S Scrutton; David Leys; . 2010: Large-scale domain dynamics and adenosylcobalamin reorientation orchestrate radical catalysis in ornithine 4,5-aminomutase. The Journal of Biological Chemistry, 285 13942-50.
- Nuria Fonknechten; Sébastien Chaussonnerie; Sabine Tricot; Aurélie Lajus; Jan R Andreesen; Nadia Perchat; Eric Pelletier; Michel Gouyvenoux; Valérie Barbe; Marcel Salanoubat; Denis Le Paslier; Jean Weissenbach; Georges N Cohen; Annett Kreimeyer; . 2010: Clostridium sticklandii, a specialist in amino acid degradation:revisiting its metabolism through its genome sequence. BMC Genomics, 11 555.
- Caitlyn Makins; François N Miros; Nigel S Scrutton; Kirsten R Wolthers; . 2012: Role of histidine 225 in adenosylcobalamin-dependent ornithine 4,5-aminomutase. Bioorganic chemistry, 40 39-47.
- Jiayun Pang; Xin Li; Keiji Morokuma; Nigel S Scrutton; Michael J Sutcliffe; . 2012: Large-scale domain conformational change is coupled to the activation of the Co-C bond in the B12-dependent enzyme ornithine 4,5-aminomutase: a computational study. Journal of the American Chemical Society, 134 2367-77.
- Caitlyn Makins; Alex V Pickering; Chloe Mariani; Kirsten R Wolthers; . 2013: Mutagenesis of a conserved glutamate reveals the contribution of electrostatic energy to adenosylcobalamin co-C bond homolysis in ornithine 4,5-aminomutase and methylmalonyl-CoA mutase. Biochemistry, 52 878-88.
- Amarendra Nath Maity; Yung-Han Chen; Shyue-Chu Ke; . 2014: Large-scale domain motions and pyridoxal-5'-phosphate assisted radical catalysis in coenzyme B12-dependent aminomutases. International journal of molecular sciences, 15 3064-87.
- Jiayun Pang; Nigel S Scrutton; Michael J Sutcliffe; . 2014: Quantum mechanics/molecular mechanics studies on the mechanism of action of cofactor pyridoxal 5'-phosphate in ornithine 4,5-aminomutase. Chemistry (Weinheim an der Bergstrasse, Germany), 20 11390-401.
- Caitlyn Makins; Doug A Whitelaw; Changhua Mu; Charles J Walsby; Kirsten R Wolthers; . 2014: Isotope effects for deuterium transfer and mutagenesis of Tyr187 provide insight into controlled radical chemistry in adenosylcobalamin-dependent ornithine 4,5-aminomutase. Biochemistry, 53 5432-43.
- Binuraj R K Menon; Karl Fisher; Stephen E J Rigby; Nigel S Scrutton; David Leys; . 2014: A Conformational Sampling Model for Radical Catalysis in Pyridoxal Phosphate- and Cobalamin-dependent Enzymes. The Journal of Biological Chemistry, 289 34161-34174.
- Binuraj R K Menon; Navya Menon; Karl Fisher; Stephen E J Rigby; David Leys; Nigel S Scrutton; . 2015: Glutamate 338 is an electrostatic facilitator of C-Co bond breakage in a dynamic/electrostatic model of catalysis by ornithine aminomutase. The FEBS journal, 282 1242-55.
- Caitlyn Makins; Douglas A Whitelaw; Michael McGregor; Alix Petit; Robert G Mothersole; Kathleen E Prosser; Kirsten R Wolthers; . 2017: Optimal Electrostatic Interactions between Substrate and Protein are Essential for Radical Chemistry in Ornithine 4,5-aminomutase. Biochimica et biophysica acta, .
Cross links
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Enzyme