OENC41549 Catalytic biopart Map View
experimental evidence
Name
Organism
Staphylococcus aureus subsp. aureus Mu50
Optimum pH
Optimum temperature(ºC)
Chassis
Catalytic functions

1) (2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate + H+ + NADPH + pyruvate => H2O + NADP+ + staphylopine

staphylopine
CHEBI: 141669
Formula: C13H19N4O6
+
NADP+
CHEBI: 58349
Formula: C21H25N7O17P3
+
H2O
CHEBI: 15377
Formula: H2O
=>
H+
CHEBI : 15378
Formula: H
+
pyruvate
CHEBI : 15361
Formula: C3H3O3
+
NADPH
CHEBI : 57783
Formula: C21H26N7O17P3
+
(2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate
CHEBI : 141808
Formula: C10H16N4O4
External Links
57510

Bioparts catalyze this reaction

Biopart ID Organisms pH temperature(ºC) km

2) H2O + NADP+ + staphylopine = (2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate + H+ + NADPH + pyruvate

H2O
CHEBI: 15377
Formula: H2O
+
NADP+
CHEBI: 58349
Formula: C21H25N7O17P3
+
staphylopine
CHEBI: 141669
Formula: C13H19N4O6
=
(2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate
CHEBI : 141808
Formula: C10H16N4O4
+
H+
CHEBI : 15378
Formula: H
+
NADPH
CHEBI : 57783
Formula: C21H26N7O17P3
+
pyruvate
CHEBI : 15361
Formula: C3H3O3
External Links
57508
EC number

Bioparts catalyze this reaction

Biopart ID Organisms pH temperature(ºC) km
Qualitative and quantitative parameters:
-
-

Temperature

Temperature optimum(ºC)
-

pH

pH optimum
-
Organism

Taxonomy

Taxonomy ID
158878
Organism
Staphylococcus aureus subsp. aureus Mu50
Lineage
cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; Staphylococcus aureus; Staphylococcus aureus subsp. aureus;
Sequence and structure

Protein sequence

Protein ID
OENC41549.p
Xref
A0A0H3JT80

Sequence

TESTMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSAR静态页面数据

Protein family

Pfam entry
definition

Protein structure

PDB ID

Nucleotide sequences (Optimized nucleotide sequences according to different chassises)

NT seq ID
OENC41549.n.1
Gene name

Sequence

ASGSDGLJSLKJGOJSIDJIOJG静态数据
References
  1. M Kuroda; T Ohta; I Uchiyama; T Baba; H Yuzawa; I Kobayashi; L Cui; A Oguchi; K Aoki; Y Nagai; J Lian; T Ito; M Kanamori; H Matsumaru; A Maruyama; H Murakami; A Hosoyama; Y Mizutani-Ui; N K Takahashi; T Sawano; R Inoue; C Kaito; K Sekimizu; H Hirakawa; S Kuhara; S Goto; J Yabuzaki; M Kanehisa; A Yamashita; K Oshima; K Furuya; C Yoshino; T Shiba; M Hattori; N Ogasawara; H Hayashi; K Hiramatsu; . 2001: Whole genome sequencing of meticillin-resistant Staphylococcus aureus. Lancet (London, England), 357 1225-40.
  2. Ghassan Ghssein; Catherine Brutesco; Laurent Ouerdane; Clémentine Fojcik; Amélie Izaute; Shuanglong Wang; Christine Hajjar; Ryszard Lobinski; David Lemaire; Pierre Richaud; Romé Voulhoux; Akbar Espaillat; Felipe Cava; David Pignol; Elise Borezée-Durant; Pascal Arnoux; . 2016: Biosynthesis of a broad-spectrum nicotianamine-like metallophore in Staphylococcus aureus. Science (New York, N.Y.), 352 1105-9.
  3. Jeffrey S McFarlane; Audrey L Lamb; . 2017: Biosynthesis of an Opine Metallophore by Pseudomonas aeruginosa. Biochemistry, .
  4. Jeffrey S McFarlane; Cara L Davis; Audrey L Lamb; . 2018: Staphylopine, pseudopaline and yersinopine dehydrogenases: a structural and kinetic analysis of a new functional class of opine dehydrogenase. The Journal of Biological Chemistry, .
Brenda
1.5.1.52
Uniprot
ODH_STAAM
Rhea
57508 , 57510
Enzyme
1.5.1.52