OENC42003 Catalytic biopart Map View
experimental evidence
Name
Organism
Sulfolobus sp.
Optimum pH
Optimum temperature(ºC)
Catalytic functions

1) 2-oxoglutarate + CoA + 2 oxidized [2Fe-2S]-[ferredoxin] <=> CO2 + H+ + 2 reduced [2Fe-2S]-[ferredoxin] + succinyl-CoA

2-oxoglutarate
CHEBI: 16810
Formula: C5H4O5
+
CoA
CHEBI: 57287
Formula: C21H32N7O16P3S
+
oxidized [2Fe-2S]-[ferredoxin]
GENERIC: 10000
<=>
CO2
CHEBI : 16526
Formula: CO2
+
H+
CHEBI : 15378
Formula: H
+
reduced [2Fe-2S]-[ferredoxin]
GENERIC : 10001
+
succinyl-CoA
CHEBI : 57292
Formula: C25H35N7O19P3S
External Links
17300 R01197
EC number

Bioparts catalyze this reaction

Biopart ID Organisms pH temperature(ºC) km

2) a 2-oxocarboxylate + CoA + 2 oxidized [2Fe-2S]-[ferredoxin] <=> an acyl-CoA + CO2 + H+ + 2 reduced [2Fe-2S]-[ferredoxin]

a 2-oxocarboxylate
CHEBI: 35179
Formula: C2O3R
+
CoA
CHEBI: 57287
Formula: C21H32N7O16P3S
+
oxidized [2Fe-2S]-[ferredoxin]
GENERIC: 10000
<=>
an acyl-CoA
CHEBI : 58342
Formula: C22H31N7O17P3SR
+
CO2
CHEBI : 16526
Formula: CO2
+
H+
CHEBI : 15378
Formula: H
+
reduced [2Fe-2S]-[ferredoxin]
GENERIC : 10001
External Links
42319 R10516
EC number
  • 1.2.7.11 2-oxoacid oxidoreductase (ferredoxin).

Bioparts catalyze this reaction

Biopart ID Organisms pH temperature(ºC) km

3) a 2-oxocarboxylate + CoA + 2 oxidized [2Fe-2S]-[ferredoxin] = an acyl-CoA + CO2 + H+ + 2 reduced [2Fe-2S]-[ferredoxin]

a 2-oxocarboxylate
CHEBI: 35179
Formula: C2O3R
+
CoA
CHEBI: 57287
Formula: C21H32N7O16P3S
+
oxidized [2Fe-2S]-[ferredoxin]
GENERIC: 10000
=
an acyl-CoA
CHEBI : 58342
Formula: C22H31N7O17P3SR
+
CO2
CHEBI : 16526
Formula: CO2
+
H+
CHEBI : 15378
Formula: H
+
reduced [2Fe-2S]-[ferredoxin]
GENERIC : 10001
External Links
42316
EC number
  • 1.2.7.11 2-oxoacid oxidoreductase (ferredoxin).

Bioparts catalyze this reaction

Biopart ID Organisms pH temperature(ºC) km
Qualitative and quantitative parameters:
-

Temperature

Temperature optimum(ºC)
-

pH

pH optimum
-
Organism

Taxonomy

Taxonomy ID
2288
Organism
Sulfolobus sp.
Lineage
cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Sulfolobus; unclassified Sulfolobus;
-
Sequence and structure

Protein sequence

Protein ID
OENC42003.p
Xref
P72578

Sequence

TESTMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSAR静态页面数据

Protein family

Pfam entry
definition
References
  1. L G Ljungdahl; . 1986: The autotrophic pathway of acetate synthesis in acetogenic bacteria. Annual review of microbiology, 40 415-50.
  2. Q Zhang; T Iwasaki; T Wakagi; T Oshima; . 1996: 2-oxoacid:ferredoxin oxidoreductase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7. Journal of biochemistry, 120 587-99.
  3. E Fukuda; H Kino; H Matsuzawa; T Wakagi; . 2001: Role of a highly conserved YPITP motif in 2-oxoacid:ferredoxin oxidoreductase: heterologous expression of the gene from Sulfolobus sp.strain 7, and characterization of the recombinant and variant enzymes. European Journal of Biochemistry, 268 5639-46.
  4. Javier Seravalli; Manoj Kumar; Stephen W Ragsdale; . 2002: Rapid kinetic studies of acetyl-CoA synthesis: evidence supporting the catalytic intermediacy of a paramagnetic NiFeC species in the autotrophic Wood-Ljungdahl pathway. Biochemistry, 41 1807-19.
  5. Eriko Fukuda; Takayoshi Wakagi; . 2002: Substrate recognition by 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus sp. strain 7. Biochimica et biophysica acta, 1597 74-80.
  6. Michael Hügler; Carl O Wirsen; Georg Fuchs; Craig D Taylor; Stefan M Sievert; . 2005: Evidence for autotrophic CO2 fixation via the reductive tricarboxylic acid cycle by members of the epsilon subdivision of proteobacteria. Journal of Bacteriology, 187 3020-7.
  7. Harald Huber; Martin Gallenberger; Ulrike Jahn; Eva Eylert; Ivan A Berg; Daniel Kockelkorn; Wolfgang Eisenreich; Georg Fuchs; . 2008: A dicarboxylate/4-hydroxybutyrate autotrophic carbon assimilation cycle in the hyperthermophilic Archaeum Ignicoccus hospitalis. Proceedings of the National Academy of Sciences of the United States of America, 105 7851-6.
  8. Robin Teufel; Johannes W Kung; Daniel Kockelkorn; Birgit E Alber; Georg Fuchs; . 2009: 3-hydroxypropionyl-coenzyme A dehydratase and acryloyl-coenzyme A reductase, enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in the Sulfolobales. Journal of Bacteriology, 191 4572-81.
  9. Jan Zarzycki; Volker Brecht; Michael Müller; Georg Fuchs; . 2009: Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus. Proceedings of the National Academy of Sciences of the United States of America, 106 21317-22.
Brenda
1.2.7.11
Uniprot
OFOA_SULSP
KEGG
BAA10898
Rhea
42316
Enzyme
1.2.7.11