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Escherichia coli C43 (DE3) cells , Escherichia coli (DE3)
1) a 2-oxocarboxylate + CoA + 2 oxidized [2Fe-2S]-[ferredoxin] = an acyl-CoA + CO2 + H+ + 2 reduced [2Fe-2S]-[ferredoxin]
oxidized [2Fe-2S]-[ferredoxin]
reduced [2Fe-2S]-[ferredoxin]
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1.2.7.11 2-oxoacid oxidoreductase (ferredoxin).
Bioparts catalyze this reaction
Biopart ID | Organisms | pH | temperature(ºC) | km |
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Qualitative and quantitative parameters:
Temperature
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80.0
pH
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8.5
Taxonomy
Protein sequence
Sequence
Protein family
Protein structure
Nucleotide sequences (Optimized nucleotide sequences according to different chassises)
Sequence
- L Kerscher; D Oesterhelt; . 1981: Purification and properties of two 2-oxoacid:ferredoxin oxidoreductases from Halobacterium halobium. European Journal of Biochemistry, 116 587-94.
- Q Zhang; T Iwasaki; T Wakagi; T Oshima; . 1996: 2-oxoacid:ferredoxin oxidoreductase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7. Journal of biochemistry, 120 587-99.
- T Iwasaki; T Oshima; . 2001: Ferredoxin and related enzymes from Sulfolobus. Methods in enzymology, 334 3-22.
- Y Kawarabayasi; Y Hino; H Horikawa; K Jin-no; M Takahashi; M Sekine; S Baba; A Ankai; H Kosugi; A Hosoyama; S Fukui; Y Nagai; K Nishijima; R Otsuka; H Nakazawa; M Takamiya; Y Kato; T Yoshizawa; T Tanaka; Y Kudoh; J Yamazaki; N Kushida; A Oguchi; K Aoki; S Masuda; M Yanagii; M Nishimura; A Yamagishi; T Oshima; H Kikuchi; . 2001: Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7. DNA research : an international journal for rapid publication of reports on genes and genomes, 8 123-40.
- E Fukuda; H Kino; H Matsuzawa; T Wakagi; . 2001: Role of a highly conserved YPITP motif in 2-oxoacid:ferredoxin oxidoreductase: heterologous expression of the gene from Sulfolobus sp.strain 7, and characterization of the recombinant and variant enzymes. European Journal of Biochemistry, 268 5639-46.
- Yumie Nishizawa; Takashi Yabuki; Eriko Fukuda; Takayoshi Wakagi; . 2005: Gene expression and characterization of two 2-oxoacid:ferredoxin oxidoreductases from Aeropyrum pernix K1. Febs Letters, 579 2319-22.
- Young-Jun Park; Chul-Bae Yoo; Soo Young Choi; Hee-Bong Lee; . 2006: Purifications and characterizations of a ferredoxin and its related 2-oxoacid:ferredoxin oxidoreductase from the hyperthermophilic archaeon, Sulfolobus solfataricus P1. Journal of biochemistry and molecular biology, 39 46-54.
- Zhen Yan; Shinya Fushinobu; Takayoshi Wakagi; . 2014: Four Cys residues in heterodimeric 2-oxoacid:ferredoxin oxidoreductase are required for CoA-dependent oxidative decarboxylation but not for a non-oxidative decarboxylation. Biochimica et biophysica acta, 1844 736-43.
- Marcus I Gibson; Percival Yang-Ting Chen; Catherine L Drennan; . 2016: A structural phylogeny for understanding 2-oxoacid oxidoreductase function. Current opinion in structural biology, 41 54-61.
- Zhen Yan; Akane Maruyama; Takatoshi Arakawa; Shinya Fushinobu; Takayoshi Wakagi; . 2016: Crystal structures of archaeal 2-oxoacid:ferredoxin oxidoreductases from Sulfolobus tokodaii. Scientific Reports, 6 33061.