OENC42060
Catalytic biopart
Map View
experimental evidence
Organism
Chassis
-
Pichia pastoris
Catalytic functions
1) Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor
EC number
-
2.4.1.221 Peptide-O-fucosyltransferase.
Bioparts catalyze this reaction
| Biopart ID | Organisms | pH | temperature(ºC) | km |
|---|
Qualitative and quantitative parameters:
Temperature
Temperature optimum(ºC)
-
25.0
pH
pH optimum
-
7.5
Organism
Taxonomy
Taxonomy ID
Organism
Lineage
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis;
Sequence and structure
Protein sequence
Protein ID
Sequence
TESTMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSAR静态页面数据
Protein family
definition
Protein structure
References
- Y Wang; G F Lee; R F Kelley; M W Spellman; . 1996: Identification of a GDP-L-fucose:polypeptide fucosyltransferase and enzymatic addition of O-linked fucose to EGF domains. Glycobiology, 6 837-42.
- Y Wang; M W Spellman; . 1998: Purification and characterization of a GDP-fucose:polypeptide fucosyltransferase from Chinese hamster ovary cells. The Journal of Biological Chemistry, 273 8112-8.
- ; . 1998: Genome sequence of the nematode C. elegans: a platform for investigating biology. Science (New York, N.Y.), 282 2012-8.
- Y Wang; L Shao; S Shi; R J Harris; M W Spellman; P Stanley; R S Haltiwanger; . 2001: Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase. The Journal of Biological Chemistry, 276 40338-45.
- Shaolin Shi; Pamela Stanley; . 2003: Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways. Proceedings of the National Academy of Sciences of the United States of America, 100 5234-9.
- Tetsuya Okajima; Aiguo Xu; Kenneth D Irvine; . 2003: Modulation of notch-ligand binding by protein O-fucosyltransferase 1 and fringe. The Journal of Biological Chemistry, 278 42340-5.
- Takeshi Sasamura; Nobuo Sasaki; Fumiyasu Miyashita; Shiho Nakao; Hiroyuki O Ishikawa; Mikiko Ito; Motoo Kitagawa; Kenichi Harigaya; Eric Spana; David Bilder; Norbert Perrimon; Kenji Matsuno; . 2003: neurotic, a novel maternal neurogenic gene, encodes an O-fucosyltransferase that is essential for Notch-Delta interactions. Development (Cambridge, England), 130 4785-95.
- Yi Luo; Robert S Haltiwanger; . 2005: O-fucosylation of notch occurs in the endoplasmic reticulum. The Journal of Biological Chemistry, 280 11289-94.
- N J Speer; S-J Tang; T Miller; T-C Chiang; . 2006: Coherent electronic fringe structure in incommensurate silver-silicon quantum wells. Science (New York, N.Y.), 314 804-6.
- Nobuo Sasaki; Takeshi Sasamura; Hiroyuki O Ishikawa; Maiko Kanai; Ryu Ueda; Kaoru Saigo; Kenji Matsuno; . 2007: Polarized exocytosis and transcytosis of Notch during its apical localization in Drosophila epithelial cells. Genes to cells : devoted to molecular & cellular mechanisms, 12 89-103.
- Takeshi Sasamura; Hiroyuki O Ishikawa; Nobuo Sasaki; Syunsuke Higashi; Maiko Kanai; Shiho Nakao; Tomonori Ayukawa; Toshiro Aigaki; Katsuhisa Noda; Eiji Miyoshi; Naoyuki Taniguchi; Kenji Matsuno; . 2007: The O-fucosyltransferase O-fut1 is an extracellular component that is essential for the constitutive endocytic trafficking of Notch in Drosophila. Development (Cambridge, England), 134 1347-56.
- Yuko Chigira; Takuji Oka; Tetsuya Okajima; Yoshifumi Jigami; . 2008: Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domains. Glycobiology, 18 303-14.
- Tetsuya Okajima; Aiko Matsuura; Tsukasa Matsuda; . 2008: Biological functions of glycosyltransferase genes involved in O-fucose glycan synthesis. Journal of biochemistry, 144 1-6.
- Nicolas Vodovar; François Schweisguth; . 2008: Functions of O-fucosyltransferase in Notch trafficking and signaling: towards the end of a controversy? Journal of biology, 7 7.
- Mi-Lyang Kim; Kumaran Chandrasekharan; Matthew Glass; Shaolin Shi; Mark C Stahl; Brian Kaspar; Pamela Stanley; Paul T Martin; . 2008: O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. Molecular and cellular neurosciences, 39 452-64.
- Pamela Stanley; Cynthia J Guidos; . 2009: Regulation of Notch signaling during T- and B-cell development by O-fucose glycans. Immunological reviews, 230 201-15.
- David Yao; Yuanshuai Huang; Xiaoran Huang; Weihuan Wang; Quanjian Yan; Lebing Wei; Wei Xin; Stanton Gerson; Pamela Stanley; John B Lowe; Lan Zhou; . 2011: Protein O-fucosyltransferase 1 (Pofut1) regulates lymphoid and myeloid homeostasis through modulation of Notch receptor ligand interactions. Blood, 117 5652-62.
- Nadia A Rana; Robert S Haltiwanger; . 2011: Fringe benefits: functional and structural impacts of O-glycosylation on the extracellular domain of Notch receptors. Current opinion in structural biology, 21 583-9.
- Erandi Lira-Navarrete; Jessika Valero-González; Raquel Villanueva; Marta Martínez-Júlvez; Tomás Tejero; Pedro Merino; Santosh Panjikar; Ramon Hurtado-Guerrero; . 2011: Structural insights into the mechanism of protein O-fucosylation. PLoS ONE, 6 e25365.
- Chun-I Chen; Jeremy J Keusch; Dominique Klein; Daniel Hess; Jan Hofsteenge; Heinz Gut; . 2012: Structure of human POFUT2: insights into thrombospondin type 1 repeat fold and O-fucosylation. The EMBO journal, 31 3183-97.
- Audrey Der Vartanian; Aymeric Audfray; Bilal Al Jaam; Mathilde Janot; Sébastien Legardinier; Abderrahman Maftah; Agnès Germot; . 2014: Protein O-fucosyltransferase 1 expression impacts myogenic C2C12 cell commitment via Notch signaling pathway. Molecular and cellular biology, 35 391-405.
- Jessika Valero-González; Christina Leonhard-Melief; Erandi Lira-Navarrete; Gonzalo Jiménez-Osés; Cristina Hernández-Ruiz; María Carmen Pallarés; Inmaculada Yruela; Deepika Vasudevan; Anabel Lostao; Francisco Corzana; Hideyuki Takeuchi; Robert S Haltiwanger; Ramon Hurtado-Guerrero; . 2016: A proactive role of water molecules in acceptor recognition by protein O-fucosyltransferase 2. Nature chemical biology, .
Cross links
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