OENC42081
Catalytic biopart
Map View
experimental evidence
Organism
Chassis
-
Pichia pastoris
Catalytic functions
1) Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor
EC number
-
2.4.1.221 Peptide-O-fucosyltransferase.
Bioparts catalyze this reaction
| Biopart ID | Organisms | pH | temperature(ºC) | km |
|---|
Qualitative and quantitative parameters:
Organism
Taxonomy
Taxonomy ID
Organism
Lineage
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis;
Sequence and structure
Protein sequence
Protein ID
Sequence
TESTMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSARQLLARLDARPLAARAAVDVAALVRRAGATLRLRRKEAVSVLDSADIEVTDSRLPHATIVDHRPQHRWLETCNAPPQLIQGKAHSAPKPSQASGKAHSAPKPSQASGAMEPRTGGAANPKGSRGSRGPSPLAGPSAR静态页面数据
Protein family
definition
Protein structure
Nucleotide sequences (Optimized nucleotide sequences according to different chassises)
NT seq ID
Sequence
ASGSDGLJSLKJGOJSIDJIOJG静态数据
References
- Y Wang; G F Lee; R F Kelley; M W Spellman; . 1996: Identification of a GDP-L-fucose:polypeptide fucosyltransferase and enzymatic addition of O-linked fucose to EGF domains. Glycobiology, 6 837-42.
- Y Wang; M W Spellman; . 1998: Purification and characterization of a GDP-fucose:polypeptide fucosyltransferase from Chinese hamster ovary cells. The Journal of Biological Chemistry, 273 8112-8.
- ; . 1998: Genome sequence of the nematode C. elegans: a platform for investigating biology. Science (New York, N.Y.), 282 2012-8.
- Olivier Menzel; Tibor Vellai; Krisztina Takacs-Vellai; Alexandre Reymond; Fritz Mueller; Stylianos E Antonarakis; Michel Guipponi; . 2004: The Caenorhabditis elegans ortholog of C21orf80, a potential new protein O-fucosyltransferase, is required for normal development. Genomics, 84 320-30.
- Hiroyuki Kaji; Jun-Ichi Kamiie; Hirotaka Kawakami; Kazuki Kido; Yoshio Yamauchi; Takashi Shinkawa; Masato Taoka; Nobuhiro Takahashi; Toshiaki Isobe; . 2007: Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins. Molecular & cellular proteomics : MCP, 6 2100-9.
- Tetsuya Okajima; Aiko Matsuura; Tsukasa Matsuda; . 2008: Biological functions of glycosyltransferase genes involved in O-fucose glycan synthesis. Journal of biochemistry, 144 1-6.
- Nicolas Vodovar; François Schweisguth; . 2008: Functions of O-fucosyltransferase in Notch trafficking and signaling: towards the end of a controversy? Journal of biology, 7 7.
- Mark Stahl; Kazuhide Uemura; Changhui Ge; Shaolin Shi; Yuko Tashima; Pamela Stanley; . 2008: Roles of Pofut1 and O-fucose in mammalian Notch signaling. The Journal of Biological Chemistry, 283 13638-51.
- Yoshiaki Okamura; Yumiko Saga; . 2008: Pofut1 is required for the proper localization of the Notch receptor during mouse development. Mechanisms of development, 125 663-73.
- Hiroyuki O Ishikawa; Tomonori Ayukawa; Minoru Nakayama; Shunsuke Higashi; Shin Kamiyama; Shoko Nishihara; Kazuhisa Aoki; Nobuhiro Ishida; Yutaka Sanai; Kenji Matsuno; . 2010: Two pathways for importing GDP-fucose into the endoplasmic reticulum lumen function redundantly in the O-fucosylation of Notch in Drosophila. The Journal of Biological Chemistry, 285 4122-9.
- Nadia A Rana; Robert S Haltiwanger; . 2011: Fringe benefits: functional and structural impacts of O-glycosylation on the extracellular domain of Notch receptors. Current opinion in structural biology, 21 583-9.
- Erandi Lira-Navarrete; Jessika Valero-González; Raquel Villanueva; Marta Martínez-Júlvez; Tomás Tejero; Pedro Merino; Santosh Panjikar; Ramon Hurtado-Guerrero; . 2011: Structural insights into the mechanism of protein O-fucosylation. PLoS ONE, 6 e25365.
- Chun-I Chen; Jeremy J Keusch; Dominique Klein; Daniel Hess; Jan Hofsteenge; Heinz Gut; . 2012: Structure of human POFUT2: insights into thrombospondin type 1 repeat fold and O-fucosylation. The EMBO journal, 31 3183-97.
- Esam Al-Shareffi; Jean-Luc Chaubard; Christina Leonhard-Melief; Sheng-Kai Wang; Chi-Huey Wong; Robert S Haltiwanger; . 2013: 6-alkynyl fucose is a bioorthogonal analog for O-fucosylation of epidermal growth factor-like repeats and thrombospondin type-1 repeats by protein O-fucosyltransferases 1 and 2. Glycobiology, 23 188-98.
- Satoshi Yokota; Katsunori Ogawara; Ryota Kimura; Fumie Shimizu; Takao Baba; Yasuyuki Minakawa; Morihiro Higo; Atsushi Kasamatsu; Yosuke Endo-Sakamoto; Masashi Shiiba; Hideki Tanzawa; Katsuhiro Uzawa; . 2013: Protein O-fucosyltransferase 1: a potential diagnostic marker and therapeutic target for human oral cancer. International journal of oncology, 43 1864-70.
- Audrey Der Vartanian; Aymeric Audfray; Bilal Al Jaam; Mathilde Janot; Sébastien Legardinier; Abderrahman Maftah; Agnès Germot; . 2014: Protein O-fucosyltransferase 1 expression impacts myogenic C2C12 cell commitment via Notch signaling pathway. Molecular and cellular biology, 35 391-405.
- Jessika Valero-González; Christina Leonhard-Melief; Erandi Lira-Navarrete; Gonzalo Jiménez-Osés; Cristina Hernández-Ruiz; María Carmen Pallarés; Inmaculada Yruela; Deepika Vasudevan; Anabel Lostao; Francisco Corzana; Hideyuki Takeuchi; Robert S Haltiwanger; Ramon Hurtado-Guerrero; . 2016: A proactive role of water molecules in acceptor recognition by protein O-fucosyltransferase 2. Nature chemical biology, .
- Lijie Ma; Pingping Dong; Longzi Liu; Qiang Gao; Meng Duan; Si Zhang; She Chen; Ruyi Xue; Xiaoying Wang; . 2016: Overexpression of protein O-fucosyltransferase 1 accelerates hepatocellular carcinoma progression via the Notch signaling pathway. Biochemical and Biophysical Research Communications, .
Cross links
Brenda
Uniprot
Enzyme