Detail
Experiment ID:
EXP00036
Reference:
- Title: MicroRNA expression profiling of human breast cancer identifies new markers of tumor subtype.
- Author: Blenkiron C, Goldstein LD, Thorne NP, Spiteri I, Chin SF, Dunning MJ, Barbosa-Morais NL, Teschendorff AE, Green AR, Ellis IO, Tavaré S, Caldas C, Miska EA
- Journal: Genome biology.2007;8(10):R214.doi:10.1186/gb-2007-8-10-r214.
- Abstract: MicroRNAs (miRNAs), a class of short non-coding RNAs found in many plants and animals, often act post-transcriptionally to inhibit gene expression., Here we report the analysis of miRNA expression in 93 primary human breast tumors, using a bead-based flow cytometric miRNA expression profiling method. Of 309 human miRNAs assayed, we identify 133 miRNAs expressed in human breast and breast tumors. We used mRNA expression profiling to classify the breast tumors as luminal A, luminal B, basal-like, HER2+ and normal-like. A number of miRNAs are differentially expressed between these molecular tumor subtypes and individual miRNAs are associated with clinicopathological factors. Furthermore, we find that miRNAs could classify basal versus luminal tumor subtypes in an independent data set. In some cases, changes in miRNA expression correlate with genomic loss or gain; in others, changes in miRNA expression are likely due to changes in primary transcription and or miRNA biogenesis. Finally, the expression of DICER1 and AGO2 is correlated with tumor subtype and may explain some of the changes in miRNA expression observed., This study represents the first integrated analysis of miRNA expression, mRNA expression and genomic changes in human breast cancer and may serve as a basis for functional studies of the role of miRNAs in the etiology of breast cancer. Furthermore, we demonstrate that bead-based flow cytometric miRNA expression profiling might be a suitable platform to classify breast cancer into prognostic molecular subtypes.
- PMID: 17922911
Expression Profile:
- Description:MicroRNA expression profiling of human breast cancer identifies new markers of tumour subtype
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE7842
- Platform: GPL5173
- Number of samples:119
- Overall design:miRNA expression data for 93 primary human breast tumours, 21 cell lines and five normal breast samples
- Instrument:Bead-based microRNA profiling platform version 3
Design and Sample:
- Cancer Type:breast cancer
- Cancer SubType:ER negative
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:ER negative breast tumour
- Control Sample:normal breast
- Num of Case:33
- Num of Control:5
- Quantification Software:Limma
- Num of miRNAs:137
Identification:
- Num of Up:14
- Num of Down:16
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|