Detail
Experiment ID:
EXP00051
Reference:
- Title: Diagnostic and prognostic implications of microRNAs in human hepatocellular carcinoma.
- Author: Li W, Xie L, He X, Li J, Tu K, Wei L, Wu J, Guo Y, Ma X, Zhang P, Pan Z, Hu X, Zhao Y, Xie H, Jiang G, Chen T, Wang J, Zheng S, Cheng J, Wan D, Yang S, Li Y, Gu J
- Journal: International journal of cancer.2008 Oct 01;123(7):1616-22.doi:10.1002/ijc.23693.
- Abstract: MicroRNAs (miRNAs) are important gene regulators, which are often deregulated in cancers. In this study, the authors analyzed the microRNAs profiles of 78 matched cancer/noncanerous liver tissues from HCC patients and 10 normal liver tissues and found that 69 miRNAs were differentially expressed between hepatocellular carcinoma (HCC) and corresponding noncancerous liver tissues (N). Then the expressions of 8 differentially expressed miRNAs were validated by real time RT PCR. The set of differentially expressed miRNAs could distinctly classify HCC, N and normal liver tissues (NL). Moreover, some of these differentially expressed miRNAs were related to the clinical factors of HCC patients. Most importantly, Kaplan-Meier estimates and the log-rank test showed that high expression of hsa-miR-125b was correlated with good survival of HCC patients (hazard ratio, 1.787, 95% confidence interval, 1.020-3.133, p = 0.043). The transfection assay showed that overexpression of miR-125b in HCC cell line could obviously suppress the cell growth and phosporylation of Akt. In conclusion, the authors have demonstrated the diagnostic miRNA profile for HCC, and for the first time, identified the miR-125b with predictive significance for HCC prognosis.
- PMID: 18649363
Expression Profile:
- Description:MicroRNAs expression profile in human hepatocellular carcinoma
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE10694
- Platform: GPL6542
- Number of samples:166
- Overall design:In this study, the microRNAs expression profile in liver cancer tissue and corresponding non-cancerous tissues of 78 HCC cases followed up for up to 68 month, and in 10 normal liver tissue were analyzed by using microarray probed with 509 miRNAs. The analysis suggested one profile including 69 microRNAs expressed differentially between liver cancer tissues and noncacerous tissue, and some of micRNAs related to clinical factor, especially one single could be used to predict the prognosis of liver cancer patients.
- Instrument:CapitalBio Mammalian miRNA Array Services V1[1].0
Design and Sample:
- Cancer Type:hepatocellular carcinoma
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:hepatocellular carcinoma
- Control Sample:normal liver
- Num of Case:78
- Num of Control:88
- Quantification Software:Limma
- Num of miRNAs:110
Identification:
- Num of Up:30
- Num of Down:33
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|