Detail
Experiment ID:
EXP00054
Reference:
- Title: The E2F3-Oncomir-1 axis is activated in Wilms' tumor.
- Author: Kort EJ, Farber L, Tretiakova M, Petillo D, Furge KA, Yang XJ, Cornelius A, Teh BT
- Journal: Cancer research.2008 Jun 01;68(11):4034-8.doi:10.1158/0008-5472.CAN-08-0592.
- Abstract: "Oncomir-1 is an oncogenic cluster of microRNAs (miRNA) located on chromosome 13. Previous in vitro studies showed that it is transcriptionally regulated by the transcription factor E2F3. In this report, we combine expression profiling of both mRNA and miRNAs in Wilms tumor (WT) samples to provide the first evidence that the E2F3-Oncomir-1 axis, previously identified in cell culture, is deregulated in primary human tumors. Analysis of RNA expression signatures showed that an E2F3 gene signature was activated in all WT samples analyzed, in contrast to other kidney tumors. This finding was validated by immunohistochemistry on the protein level. Expression of E2F3 was lowest in early-stage tumors and highest in metastatic tissue. Expression profiling of miRNAs in WT showed that expression of each measured member of the Oncomir-1 family was highest in WT relative to other kidney tumor subtypes. Quantitative PCR confirmed that these miRNAs were overexpressed in WT relative to normal kidney tissue. These results suggest that the E2F3-Oncomir-1 axis is activated in WT. Our study also shows the utility of integrated genomics combining gene signature analysis with miRNA expression profiling to identify protein-miRNA interactions that are perturbed in disease states."
- PMID: 18519660
Expression Profile:
- Description:miRNA profiling of adult and pediatric renal tumors
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE11016
- Platform: GPL6668
- Number of samples:32
- Overall design:Samples from Clear Cell RCC, Papillary RCC, Chromophobe RCC, Oncocytoma, and Wilms Tumor compared to pooled normal kidney samples from these same patients. No technical replicates.
- Instrument:MRA-1001 Human miRNA Array versions 7, 9, and 10.1_071219
Design and Sample:
- Cancer Type:kidney cancer
- Cancer SubType:Wilms tumor
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:Wilms tumor
- Control Sample:normal kidney
- Num of Case:12
- Num of Control:12
- Quantification Software:Limma
- Num of miRNAs:148
Identification:
- Num of Up:47
- Num of Down:56
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|