Detail
Experiment ID:
EXP00075
Reference:
- Title: MicroRNA expression signatures in Barrett's esophagus and esophageal adenocarcinoma.
- Author: Yang H, Gu J, Wang KK, Zhang W, Xing J, Chen Z, Ajani JA, Wu X
- Journal: Clinical cancer research : an official journal of the American Association for Cancer Research.2009 Sep 15;15(18):5744-52.doi:10.1158/1078-0432.CCR-09-0385.
- Abstract: "Esophageal adenocarcinoma is a highly aggressive malignancy that frequently develops from Barretts esophagus, a premalignant pathologic change occurring in the lower end of the esophagus. Identifying Barretts esophagus patients at high risk of malignant transformation is essential to the prevention of esophageal adenocarcinoma. Although microRNA (miRNA) expression signatures have been associated with the etiology and prognosis of several types of cancers, their roles in the development of esophageal adenocarcinoma have not been extensively evaluated.", "In this study, we analyzed the expression patterns of 470 human miRNAs using Agilent miRNA microarray in 32 disease/normal-paired tissues from 16 patients diagnosed with Barretts esophagus of either low- or high-grade dysplasia, or esophageal adenocarcinoma.", "Using unsupervised hierarchical clustering and class comparison analyses, we found that miRNA expression profiles in tissues of Barretts esophagus with high-grade dysplasia were significantly different from their corresponding normal tissues. Similar findings were observed for esophageal adenocarcinoma, but not for Barretts esophagus with low-grade dysplasia. The expression patterns of selected miRNAs were further validated using quantitative reverse transcription real-time PCR in an independent set of 75 pairs of disease/normal tissues. Finally, we identified several miRNAs that were involved in the progressions from low grade-dysplasia Barretts esophagus to esophageal adenocarcinoma.", We showed that miRNAs were involved in the development and progression of esophageal adenocarcinoma. The identified significant miRNAs that may become potential targets for early detection, chemoprevention, and treatment of esophageal cancer.
- PMID: 19737949
Expression Profile:
- Description:MicroRNA expression signatures in Barrett's esophagus and esophageal adenocarcinoma
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE16456
- Platform: GPL6955
- Number of samples:32
- Overall design:We analyzed the expression patterns of 470 human miRNAs using Agilent miRNA microarray in 32 disease/normal-paired tissues from 16 patients diagnosed with BE of either low or high grade dysplasia, or EAC
- Instrument:Agilent-016436 Human miRNA Microarray 1.0 (Feature Number version)
Design and Sample:
- Cancer Type:esophageal cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:esophageal cancer
- Control Sample:normal esophagus
- Num of Case:6
- Num of Control:6
- Quantification Software:Limma
- Num of miRNAs:318
Identification:
- Num of Up:44
- Num of Down:69
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|