Detail
Experiment ID:
EXP00078
Reference:
- Title: MicroRNA expression profiling in human Barrett's carcinogenesis.
- Author: Fassan M, Volinia S, Palatini J, Pizzi M, Baffa R, De Bernard M, Battaglia G, Parente P, Croce CM, Zaninotto G, Ancona E, Rugge M
- Journal: International journal of cancer.2011 Oct 01;129(7):1661-70.doi:10.1002/ijc.25823.
- Abstract: "Barretts esophagus (BE) is characterized by the native stratified squamous epithelium (N) lining the esophagus being replaced by a columnar epithelium with intestinal differentiation (Barretts mucosa; BM). BM is considered as the main risk factor for esophageal adenocarcinoma (Barretts adenocarcinoma; BAc). MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting messenger RNAs and they are reportedly dysregulated in BM. To test the hypothesis that a specific miRNA expression signature characterizes BM development and progression, we performed miRNA microarray analysis comparing native esophageal mucosa with all the phenotypic lesions seen in the Barretts carcinogenic process. Specimens were collected from 14 BE patients who had undergone esophagectomy, including: 14 with N, 14 with BM, 7 with low-grade intraepithelial neoplasia, 5 with high-grade intra-epithelial neoplasia and 11 with BAc. Microarray findings were further validated by quantitive real-time polymerase chain reaction and in situ hybridization analyses using a different series of consecutive cases (162 biopsy samples and 5 esophagectomies) of histologically proven, long-segment BE. We identified a miRNA signature of Barretts carcinogenesis consisting of an increased expression of 6 miRNAs and a reduced expression of 7 miRNAs. To further support these results, we investigated target gene expression using the Oncomine database and/or immunohistochemical analysis. We found that target gene expression correlated significantly with miRNA dysregulation. Specific miRNAs are directly involved in BE progression to cancer. miRNA profiling significantly expands current knowledge on the molecular history of Barretts carcinogenesis, also identifying molecular markers of cancer progression."
- PMID: 21128279
Expression Profile:
- Description:microRNA/UCR expression profiling of human Barrett's carcinogenesis
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE20099
- Platform: GPL8871
- Number of samples:51
- Overall design:miRNA/UCR microarray analysis was performed in a series of 14 normal esophageal squamous epithelium samples, 14 Barrett's mucosa samples, 7 low-grade intra-epithelial neoplasia samples, 5 high-grade intra-epithelial neoplasia samples and 11 Barrett's adenocarcinoma samples
- Instrument:OSU_CCC v4.0 [condensed version]
Design and Sample:
- Cancer Type:esophageal cancer
- Cancer SubType:Barrett's carcinogenesis
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:Barrett's adenocarcinoma
- Control Sample:normal esophagus
- Num of Case:11
- Num of Control:14
- Quantification Software:Limma
- Num of miRNAs:605
Identification:
- Num of Up:41
- Num of Down:28
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|