Detail
Experiment ID:
EXP00089
Reference:
- Title: MicroRNA-193b represses cell proliferation and regulates cyclin D1 in melanoma.
- Author: Chen J, Feilotter HE, Paré GC, Zhang X, Pemberton JG, Garady C, Lai D, Yang X, Tron VA
- Journal: The American journal of pathology.2010 May ;176(5):2520-9.doi:10.2353/ajpath.2010.091061.
- Abstract: "Cutaneous melanoma is an aggressive form of human skin cancer characterized by high metastatic potential and poor prognosis. To better understand the role of microRNAs (miRNAs) in melanoma, the expression of 470 miRNAs was profiled in tissue samples from benign nevi and metastatic melanomas. We identified 31 miRNAs that were differentially expressed (13 up-regulated and 18 down-regulated) in metastatic melanomas relative to benign nevi. Notably, miR-193b was significantly down-regulated in the melanoma tissues examined. To understand the role of miR-193b in melanoma, functional studies were undertaken. Overexpression of miR-193b in melanoma cell lines repressed cell proliferation. Gene expression profiling identified 314 genes down-regulated by overexpression of miR-193b in Malme-3M cells. Eighteen of these down-regulated genes, including cyclin D1 (CCND1), were also identified as putative miR-193b targets by TargetScan. Overexpression of miR-193b in Malme-3M cells down-regulated CCND1 mRNA and protein by > or = 50%. A luciferase reporter assay confirmed that miR-193b directly regulates CCND1 by binding to the 3untranslated region of CCND1 mRNA. These studies indicate that miR-193b represses cell proliferation and regulates CCND1 expression and suggest that dysregulation of miR-193b may play an important role in melanoma development."
- PMID: 20304954
Expression Profile:
- Description:miR-193b represses cell proliferation and regulates cyclin D1 in melanoma: benign nevi and metastatic melanoma
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE18509
- Platform: GPL9081
- Number of samples:16
- Overall design:8 benign nevi and 8 metastatic melanoma tissue samples were profiled by Agilent MicroRNA Microarray (V1.5).
- Instrument:Agilent-016436 Human miRNA Microarray 1.0 G4472A (miRNA ID version)
Design and Sample:
- Cancer Type:melanoma
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:metastasis
- Case Sample:metastatic melanoma
- Control Sample:benign nevi
- Num of Case:8
- Num of Control:8
- Quantification Software:Limma
- Num of miRNAs:278
Identification:
- Num of Up:37
- Num of Down:25
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|