Detail
Experiment ID:
EXP00123
Reference:
- Title: The role of microRNA deregulation in the pathogenesis of adrenocortical carcinoma.
- Author: Özata DM, Caramuta S, Velázquez-Fernández D, Akçakaya P, Xie H, Höög A, Zedenius J, Bäckdahl M, Larsson C, Lui WO
- Journal: Endocrine-related cancer.2011 Oct ;18(6):643-55.doi:10.1530/ERC-11-0082.
- Abstract: Adrenocortical carcinoma (ACC) is an aggressive tumor showing frequent metastatic spread and poor survival. Although recent genome-wide studies of ACC have contributed to our understanding of the disease, major challenges remain for both diagnostic and prognostic assessments. The aim of this study was to identify specific microRNAs (miRNAs) associated with malignancy and survival of ACC patients. miRNA expression profiles were determined in a series of ACC, adenoma, and normal cortices using microarray. A subset of miRNAs showed distinct expression patterns in the ACC compared with adrenal cortices and adenomas. Among others, miR-483-3p, miR-483-5p, miR-210, and miR-21 were found overexpressed, while miR-195, miR-497, and miR-1974 were underexpressed in ACC. Inhibition of miR-483-3p or miR-483-5p and overexpression of miR-195 or miR-497 reduced cell proliferation in human NCI-H295R ACC cells. In addition, downregulation of miR-483-3p, but not miR-483-5p, and increased expression of miR-195 or miR-497 led to significant induction of cell death. Protein expression of p53 upregulated modulator of apoptosis (PUMA), a potential target of miR-483-3p, was significantly decreased in ACC, and inversely correlated with miR-483-3p expression. In addition, high expression of miR-503, miR-1202, and miR-1275 were found significantly associated with shorter overall survival among patients with ACC (P values: 0.006, 0.005, and 0.042 respectively). In summary, we identified additional miRNAs associated with ACC, elucidated the functional role of four miRNAs in the pathogenesis of ACC cells, demonstrated the potential involvement of the pro-apoptotic factor PUMA (a miR-483-3p target) in adrenocortical tumors, and found novel miRNAs associated with survival in ACC.
- PMID: 21859927
Expression Profile:
- Description:Deregulated miRNAs in adrenocortical tumors
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE22816
- Platform: GPL13347
- Number of samples:52
- Overall design:Agilent microarray platform containing 903 miRNAs was used to determine miRNA expression profiles in 4 normal cortices, 26 adenomas and 22 adrenocortical carcinomas. SAM analysis was adopted to identify significant differentially expressed miRNAs between groups. SAM survival analysis was used to determine the association between miRNA expression and survival among carcinoma cases. The expression levels of candidate prognostic miRNAs were evaluated using qRT-PCR in the same cohort of adrenocortical carcinomas and association with survival was evaluated using Kaplan Meier curves and log rank tests.
- Instrument:Agilent-025987 Human miRNA Microarray Release 14.0 G4471A (miRNA ID version)
Design and Sample:
- Cancer Type:adrenocortical cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:subtype1 vs subtype2
- Case Sample:adrenocortical adenoma
- Control Sample:adrenocortical carcinoma
- Num of Case:26
- Num of Control:22
- Quantification Software:Limma
- Num of miRNAs:211
Identification:
- Num of Up:62
- Num of Down:38
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
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