Detail
Experiment ID:
EXP00143
Reference:
- Title: Dysregulated microRNAs affect pathways and targets of biologic relevance in nasal-type natural killer/T-cell lymphoma.
- Author: Ng SB, Yan J, Huang G, Selvarajan V, Tay JL, Lin B, Bi C, Tan J, Kwong YL, Shimizu N, Aozasa K, Chng WJ
- Journal: Blood.2011 Nov 03;118(18):4919-29.doi:10.1182/blood-2011-07-364224.
- Abstract: We performed a comprehensive genome-wide miRNA expression profiling of extranodal nasal-type natural killer/T-cell lymphoma (NKTL) using formalin-fixed paraffin-embedded tissue (n = 30) and NK cell lines (n = 6) compared with normal NK cells, with the objective of understanding the pathogenetic role of miRNA deregulation in NKTL. Compared with normal NK cells, differentially expressed miRNAs in NKTL are predominantly down-regulated. Re-expression of down-regulated miRNAs, such as miR-101, miR-26a, miR26b, miR-28-5, and miR-363, reduced the growth of the NK cell line and modulated the expression of their predicted target genes, suggesting the potential functional role of the deregulated miRNAs in the oncogenesis of NKTL. Taken together, the predicted targets whose expression is inversely correlated with the expression of deregulated miRNA in NKTL are significantly enriched for genes involved in cell cycle-related, p53, and MAPK signaling pathways. We also performed immunohistochemical validation for selected target proteins and found overexpression of MUM1, BLIMP1, and STMN1 in NKTL, and notably, a corresponding increase in MYC expression. Because MYC is known to cause repression of miRNA expression, it is possible that MYC activation in NKTL may contribute to the suppression of the miRNAs regulating MUM1, BLIMP1, and STMN1.
- PMID: 21921041
Expression Profile:
- Description:miRNA expression profiling of NKTCL (Natural Killer/T-Cell Lymphoma) samples
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE31377
- Platform: GPL8227
- Number of samples:56
- Overall design:Total RNA, including miRNA, were extracted using Ambion Recoverall Kit and profiled using Agilent human miRNA V2.
- Instrument:Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version)
Design and Sample:
- Cancer Type:lymphoma
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:Natural Killer/T-Cell Lymphoma
- Control Sample:normal NK cell
- Num of Case:38
- Num of Control:12
- Quantification Software:Limma
- Num of miRNAs:723
Identification:
- Num of Up:227
- Num of Down:177
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|