Detail
Experiment ID:
EXP00149
Reference:
- Title: Polycomb-mediated loss of miR-31 activates NIK-dependent NF-κB pathway in adult T cell leukemia and other cancers.
- Author: Yamagishi M, Nakano K, Miyake A, Yamochi T, Kagami Y, Tsutsumi A, Matsuda Y, Sato-Otsubo A, Muto S, Utsunomiya A, Yamaguchi K, Uchimaru K, Ogawa S, Watanabe T
- Journal: Cancer cell.2012 Jan 17;21(1):121-35.doi:10.1016/j.ccr.2011.12.015.
- Abstract: Constitutive NF-κB activation has causative roles in adult T cell leukemia (ATL) caused by HTLV-1 and other cancers. Here, we report a pathway involving Polycomb-mediated miRNA silencing and NF-κB activation. We determine the miRNA signatures and reveal miR-31 loss in primary ATL cells. MiR-31 negatively regulates the noncanonical NF-κB pathway by targeting NF-κB inducing kinase (NIK). Loss of miR-31 therefore triggers oncogenic signaling. In ATL cells, miR-31 level is epigenetically regulated, and aberrant upregulation of Polycomb proteins contribute to miR-31 downregulation in an epigenetic fashion, leading to activation of NF-κB and apoptosis resistance. Furthermore, this emerging circuit operates in other cancers and receptor-initiated NF-κB cascade. Our findings provide a perspective involving the epigenetic program, inflammatory responses, and oncogenic signaling.
- PMID: 22264793
Expression Profile:
- Description:miRNA expression profiling in Adult T-cell Leukemia (ATL) cells and in Normal CD4+ T-cells
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE31629
- Platform: GPL7731
- Number of samples:62
- Overall design:Total RNA samples from PBMC of ATL patients (mostly HTLV-1 inefcted CD4+ T-cells) and from CD4+ T-cells from healthy donors were subjected to Cy-3 labeling followed by miRNA expression microarray analyses.
- Instrument:Agilent-019118 Human miRNA Microarray 2.0 G4470B (Feature Number version)
Design and Sample:
- Cancer Type:leukemia
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:T-cell Leukemia
- Control Sample:Normal CD4+ T-cell
- Num of Case:40
- Num of Control:22
- Quantification Software:Limma
- Num of miRNAs:719
Identification:
- Num of Up:97
- Num of Down:62
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|