Detail
Experiment ID:
EXP00154
Reference:
- Title: Microarray analysis of colorectal cancer stromal tissue reveals upregulation of two oncogenic miRNA clusters.
- Author: Nishida N, Nagahara M, Sato T, Mimori K, Sudo T, Tanaka F, Shibata K, Ishii H, Sugihara K, Doki Y, Mori M
- Journal: Clinical cancer research : an official journal of the American Association for Cancer Research.2012 Jun 01;18(11):3054-70.doi:10.1158/1078-0432.CCR-11-1078.
- Abstract: Cancer stroma plays an important role in the progression of cancer. Although alterations in miRNA expression have been explored in various kinds of cancers, the expression of miRNAs in cancer stroma has not been explored in detail., Using a laser microdissection technique, we collected RNA samples specific for epithelium or stroma from 13 colorectal cancer tissues and four normal tissues, and miRNA microarray and gene expression microarray were carried out. The expression status of miRNAs was confirmed by reverse transcriptase PCR. Furthermore, we investigated whether miRNA expression status in stromal tissue could influence the clinicopathologic factors., Oncogenic miRNAs, including two miRNA clusters, miR-17-92a and miR-106b-25 cluster, were upregulated in cancer stromal tissues compared with normal stroma. Gene expression profiles from cDNA microarray analyses of the same stromal tissue samples revealed that putative targets of these miRNA clusters, predicted by Target Scan, such as TGFBR2, SMAD2, and BMP family genes, were significantly downregulated in cancer stromal tissue. Downregulated putative targets were also found to be involved in cytokine interaction and cellular adhesion. Importantly, expression of miR-25 and miR-92a in stromal tissues was associated with a variety of clinicopathologic factors., Oncogenic miRNAs were highly expressed in cancer stroma. Although further validation is required, the finding that stromal miRNA expression levels were associated with clinicopathologic factors suggests the possibility that miRNAs in cancer stroma are crucially involved in cancer progression.
- PMID: 22452939
Expression Profile:
- Description:microRNA and Gene expression profiles in colorectal cancer stromal tissue
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE35602
- Platform: GPL8227
- Number of samples:59
- Overall design:Tissue samples from thirteen colorectal cancer tissues and four normal tissues were microdissected using Laser Microdissection System (Leica Microsystems), and RNA samples specific for stroma or epithelium were separately collected.MicroRNA and Gene expression profiles obtained from cancer stromal tissues were compared with that of normal colon stromal tissues. we used the commercially available Human Whole Genome Oligo DNA Microarray Kit (Agilent Technologies) for gene expresson analysis and microRNA array for microRNA analysis.
- Instrument:Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version)
Design and Sample:
- Cancer Type:colorectal cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:colorectal cancer
- Control Sample:normal colon
- Num of Case:17
- Num of Control:8
- Quantification Software:Limma
- Num of miRNAs:723
Identification:
- Num of Up:3
- Num of Down:19
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|