Detail
Experiment ID:
EXP00157
Reference:
- Title: MiR-155 is a liposarcoma oncogene that targets casein kinase-1α and enhances β-catenin signaling.
- Author: Zhang P, Bill K, Liu J, Young E, Peng T, Bolshakov S, Hoffman A, Song Y, Demicco EG, Terrada DL, Creighton CJ, Anderson ML, Lazar AJ, Calin GG, Pollock RE, Lev D
- Journal: Cancer research.2012 Apr 01;72(7):1751-62.doi:10.1158/0008-5472.CAN-11-3027.
- Abstract: Liposarcoma can be an aggressive, debilitating, and fatal malignancy. In this study, we identifed miRNAs associated with the differentiation status of liposarcoma to gain insight into the basis for its progression. miRNA expression profiles determined in human tumors and normal fat specimens identified a dedifferentiated tumor expression signature consisting of 35 miRNAs. Deregulated miRNA expression was confirmed in a second independent sample cohort. The miR-155 was the most overexpressed miRNA and functional investigations assigned an important role in the growth of dedifferentiated liposarcoma cell lines. Transient or stable knockdown of miR-155 retarded tumor cell growth, decreased colony formation, and induced G(1)-S cell-cycle arrest in vitro and blocked tumor growth in murine xenografts in vivo. We identified casein kinase 1α (CK1α) as a direct target of miR-155 control which enhanced β-catenin signaling and cyclin D1 expression, promoting tumor cell growth. In summary, our results point to important functions for miR-155 and β-catenin signaling in progression of liposarcoma, revealing mechanistic vulnerabilities that might be exploited for both prognostic and therapeutic purposes.
- PMID: 22350414
Expression Profile:
- Description:MicroRNAs differentially expressed in liposarcoma
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE31045
- Platform: GPL7723
- Number of samples:25
- Overall design:We performed expression profiling of microRNAs comparing liposarcomas with normal fat pad.
- Instrument:miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
Design and Sample:
- Cancer Type:sarcoma
- Cancer SubType:liposarcoma
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:liposarcoma
- Control Sample:normal fat
- Num of Case:17
- Num of Control:8
- Quantification Software:Limma
- Num of miRNAs:835
Identification:
- Num of Up:72
- Num of Down:62
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
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