Detail
Experiment ID:
EXP00161
Reference:
- Title: The miRNA-200 family and miRNA-9 exhibit differential expression in primary versus corresponding metastatic tissue in breast cancer.
- Author: Gravgaard KH, Lyng MB, Laenkholm AV, Søkilde R, Nielsen BS, Litman T, Ditzel HJ
- Journal: Breast cancer research and treatment.2012 Jul ;134(1):207-17.doi:10.1007/s10549-012-1969-9.
- Abstract: Metastases are the major cause of cancer-related deaths, but the mechanisms of the metastatic process remain poorly understood. In recent years, the involvement of microRNAs (miRNAs) in cancer has become apparent, and the objective of this study was to identify miRNAs associated with breast cancer progression. Global miRNA expression profiling was performed on 47 tumor samples from 14 patients with paired samples from primary breast tumors and corresponding lymph node and distant metastases using LNA-enhanced miRNA microarrays. The identified miRNA expression alterations were validated by real-time PCR, and tissue distribution of the miRNAs was visualized by in situ hybridization. The patients, in which the miRNA profile of the primary tumor and corresponding distant metastasis clustered in the unsupervised cluster analysis, showed significantly shorter intervals between the diagnosis of the primary tumor and distant metastasis (median 1.6 years) compared to those that did not cluster (median 11.3 years) (p<0.003). Fifteen miRNAs were identified that were significantly differentially expressed between primary tumors and corresponding distant metastases, including miR-9, miR-219-5p and four of the five members of the miR-200 family involved in epithelial-mesenchymal transition. Tumor expression of miR-9 and miR-200b were confirmed using in situ hybridization, which also verified higher expression of these miRNAs in the distant metastases versus corresponding primary tumors. Our results demonstrate alterations in miRNA expression at different stages of disease progression in breast cancer, and suggest a direct involvement of the miR-200 family and miR-9 in the metastatic process.
- PMID: 22294488
Expression Profile:
- Description:The miRNA-200 family and miRNA-9 exhibit differential expression in primary vs. corresponding metastatic tissue in breast cancer
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE37407
- Platform: GPL13703
- Number of samples:61
- Overall design:Global miRNA expression profiling was performed on 47 tumor samples from 14 patients with paired samples from primary breast tumors and corresponding lymph node and distant metastases using LNA-enhanced miRNA microarrays. The identified miRNA expression alterations were validated by real-time PCR, and tissue distribution of the miRNAs was visualized by in situ hybridization
- Instrument:Exiqon miRCURY LNA microRNA array v.11.0 Extended Version
Design and Sample:
- Cancer Type:breast cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:metastasis
- Case Sample:metastatic breast cancer
- Control Sample:primary breast cancer
- Num of Case:31
- Num of Control:30
- Quantification Software:Limma
- Num of miRNAs:834
Identification:
- Num of Up:72
- Num of Down:86
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|