Detail
Experiment ID:
EXP00183
Reference:
- Title: MiR-145 functions as a tumor suppressor by directly targeting histone deacetylase 2 in liver cancer.
- Author: Noh JH, Chang YG, Kim MG, Jung KH, Kim JK, Bae HJ, Eun JW, Shen Q, Kim SJ, Kwon SH, Park WS, Lee JY, Nam SW
- Journal: Cancer letters.2013 Jul 28;335(2):455-62.doi:10.1016/j.canlet.2013.03.003.
- Abstract: Aberrant regulation of histone deacetylase 2 (HDAC2) plays a pivotal role in the development of hepatocellular carcinoma (HCC), but, the underlying mechanism leading to HDAC2 overexpression is not well understood. We performed microRNA (miRNA) profiling analysis in a subset of HCCs, and identified four down-regulated miRNAs that may target HDAC2 in HCC. Ectopic expression of miRNA mimics evidenced that miR-145 suppresses HDAC2 expression in HCC cells. This treatment repressed cancer cell growth and recapitulated HDAC2 knockdown effects on HCC cells. In conclusion, we suggest that loss or suppression of miR-145 may cause aberrant overexpression of HDAC2 and promote HCC tumorigenesis.
- PMID: 23499894
Expression Profile:
- Description:Oncogenic Potential of SIRT7 in Human Hepatocellular Carcinoma and its Regulation by the Tumor Suppressors MiR-125a-5p and MiR-125b
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE39678
- Platform: GPL15852
- Number of samples:24
- Overall design:miRNA expression profiling of 8 normal liver and 16 HCC tissues was performed using GenoExplorer Microarray platform. 10 µg of total RNA was isolated from normal liver and HCC tissues and then labeled and hybridized to a to microRNA chip for analysis under optimized conditions. The arrays with hybridized targets were scanned using an Axon scanner and the scanned images were analyzed using GenePix® Pro 5.0 software (Axon Instruments) and spots of poor quality determined by visual inspection were also removed from further analysis. The resulting data, the average of 3 mean fluorescence signal intensities for each probe, collected from each array was submitted to the GenePlex 3.0 (Istech Inc. Seoul, Korea) database. Data were normalized using the method of quantile normalization and class-specific filtering.
- Instrument:Homo sapiens 0.5K microRNA Ca_Hu_MiRNome_v1
Design and Sample:
- Cancer Type:hepatocellular carcinoma
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:hepatocellular carcinoma
- Control Sample:normal liver
- Num of Case:16
- Num of Control:8
- Quantification Software:Limma
- Num of miRNAs:399
Identification:
- Num of Up:55
- Num of Down:46
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
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