Detail
Experiment ID:
EXP00184
Reference:
- Title: The rectal cancer microRNAome--microRNA expression in rectal cancer and matched normal mucosa.
- Author: Gaedcke J, Grade M, Camps J, Søkilde R, Kaczkowski B, Schetter AJ, Difilippantonio MJ, Harris CC, Ghadimi BM, Møller S, Beissbarth T, Ried T, Litman T
- Journal: Clinical cancer research : an official journal of the American Association for Cancer Research.2012 Sep 15;18(18):4919-30.doi:10.1158/1078-0432.CCR-12-0016.
- Abstract: miRNAs play a prominent role in a variety of physiologic and pathologic biologic processes, including cancer. For rectal cancers, only limited data are available on miRNA expression profiles, whereas the underlying genomic and transcriptomic aberrations have been firmly established. We therefore, aimed to comprehensively map the miRNA expression patterns of this disease., Tumor biopsies and corresponding matched mucosa samples were prospectively collected from 57 patients with locally advanced rectal cancers. Total RNA was extracted, and tumor and mucosa miRNA expression profiles were subsequently established for all patients. The expression of selected miRNAs was validated using semi-quantitative real-time PCR., Forty-nine miRNAs were significantly differentially expressed (log(2)-fold difference >0.5 and P < 0.001) between rectal cancer and normal rectal mucosa. The predicted targets for these miRNAs were enriched for the following pathways: Wnt, TGF-beta, mTOR, insulin, mitogen-activated protein kinase, and ErbB signaling. Thirteen of these 49 miRNAs seem to be rectal cancer-specific, and have not been previously reported for colon cancers: miR-492, miR-542-5p, miR-584, miR-483-5p, miR-144, miR-2110, miR-652, miR-375, miR-147b, miR-148a, miR-190, miR-26a/b, and miR-338-3p. Of clinical impact, miR-135b expression correlated significantly with disease-free and cancer-specific survival in an independent multicenter cohort of 116 patients., This comprehensive analysis of the rectal cancer miRNAome uncovered novel miRNAs and pathways associated with rectal cancer. This information contributes to a detailed view of this disease. Moreover, the identification and validation of miR-135b may help to identify novel molecular targets and pathways for therapeutic exploitation.
- PMID: 22850566
Expression Profile:
- Description:The rectal cancer microRNAome - a comprehensive comparative analysis of microRNA expression in rectal cancer and matched normal mucosa
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE38389
- Platform: GPL11039
- Number of samples:140
- Overall design:Paired samples from rectal tumor and matched normal samples. Included are also 2 technical replicates.
- Instrument:Exiqon miRCURY LNA microRNA array v.9.2 Extended Version
Design and Sample:
- Cancer Type:colorectal cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:rectal tumor
- Control Sample:normal trectal mucosa
- Num of Case:69
- Num of Control:71
- Quantification Software:Limma
- Num of miRNAs:581
Identification:
- Num of Up:137
- Num of Down:130
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|