Detail
Experiment ID:
EXP00190
Reference:
- Title: miR-10b*, a master inhibitor of the cell cycle, is down-regulated in human breast tumours.
- Author: Biagioni F, Bossel Ben-Moshe N, Fontemaggi G, Canu V, Mori F, Antoniani B, Di Benedetto A, Santoro R, Germoni S, De Angelis F, Cambria A, Avraham R, Grasso G, Strano S, Muti P, Mottolese M, Yarden Y, Domany E, Blandino G
- Journal: EMBO molecular medicine.2012 Nov ;4(11):1214-29.doi:10.1002/emmm.201201483.
- Abstract: Deregulated proliferation is a hallmark of cancer cells. Here, we show that microRNA-10b* is a master regulator of breast cancer cell proliferation and is downregulated in tumoural samples versus matched peritumoural counterparts. Two canonical CpG islands (5 kb) upstream from the precursor sequence are hypermethylated in the analysed breast cancer tissues. Ectopic delivery of synthetic microRNA-10b* in breast cancer cell lines or into xenograft mouse breast tumours inhibits cell proliferation and impairs tumour growth in vivo, respectively. We identified and validated in vitro and in vivo three novel target mRNAs of miR-10b* (BUB1, PLK1 and CCNA2), which play a remarkable role in cell cycle regulation and whose high expression in breast cancer patients is associated with reduced disease-free survival, relapse-free survival and metastasis-free survival when compared to patients with low expression. This also suggests that restoration of microRNA-10b* expression might have therapeutic promise.
- PMID: 23125021
Expression Profile:
- Description:miR-10b*, a master inhibitor of the cell cycle, is downregulated in human breast tumors
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE40525
- Platform: GPL8227
- Number of samples:120
- Overall design:In exploring the potential involvement of miRs in breast tumorigenesis, we profiled the miRs expression of 64 primary breast cancer patients, comprising 56 matched tumor and adjacent peritumoral breast tissues, 5 tumor and 3 peritumor unmatched tissues, using the Agilent microarray platform. For 14 samples technical replicate were also performed.
- Instrument:Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version)
Design and Sample:
- Cancer Type:breast cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:breast cancer
- Control Sample:normal tissue
- Num of Case:61
- Num of Control:59
- Quantification Software:Limma
- Num of miRNAs:723
Identification:
- Num of Up:131
- Num of Down:143
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|