Detail
Experiment ID:
EXP00205
Reference:
- Title: Targeting glioma stem cells by functional inhibition of a prosurvival oncomiR-138 in malignant gliomas.
- Author: Chan XH, Nama S, Gopal F, Rizk P, Ramasamy S, Sundaram G, Ow GS, Ivshina AV, Tanavde V, Haybaeck J, Kuznetsov V, Sampath P
- Journal: Cell reports.2012 Sep 27;2(3):591-602.doi:10.1016/j.celrep.2012.07.012.
- Abstract: Malignant gliomas are the most aggressive forms of brain tumors, associated with high rates of morbidity and mortality. Recurrence and tumorigenesis are attributed to a subpopulation of tumor-initiating glioma stem cells (GSCs) that are intrinsically resistant to therapy. Initiation and progression of gliomas have been linked to alterations in microRNA expression. Here, we report the identification of microRNA-138 (miR-138) as a molecular signature of GSCs and demonstrate a vital role for miR-138 in promoting growth and survival of bona fide tumor-initiating cells with self-renewal potential. Sequence-specific functional inhibition of miR-138 prevents tumorsphere formation in vitro and impedes tumorigenesis in vivo. We delineate the components of the miR-138 regulatory network by loss-of-function analysis to identify specific regulators of apoptosis. Finally, the higher expression of miR-138 in GSCs compared to non-neoplastic tissue and association with tumor recurrence and survival highlights the clinical significance of miR-138 as a prognostic biomarker and a therapeutic target for treatment of malignant gliomas.
- PMID: 22921398
Expression Profile:
- Description:MicroRNA-138: A Pro-survival OncomiR for Glioma Stem Cells is Associated with Tumor Recurrence [miRNA]
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE39486
- Platform: GPL15829
- Number of samples:6
- Overall design:Total RNA from Glioma Stem Cells and Neural Stem Cells were subjected to microRNA microarray analysis, 3 replicates each.
- Instrument:Exiqon miRCURY LNA microRNA array v.11.0 [miRBase v13]
Design and Sample:
- Cancer Type:brain cancer
- Cancer SubType:glioma
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:glioma
- Control Sample:normal brain
- Num of Case:3
- Num of Control:3
- Quantification Software:Limma
- Num of miRNAs:341
Identification:
- Num of Up:67
- Num of Down:71
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
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