Detail
Experiment ID:
EXP00224
Reference:
- Title: The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis.
- Author: Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, Brojigin N, Kaneoka Y, Maeda A, Kumada T, Kawada N, Kubo S, Kuroda M
- Journal: BMC cancer.2013 Mar 04;13:99.doi:10.1186/1471-2407-13-99.
- Abstract: Many studies support the hypothesis that specific microRNA (miRNA) expression in various human cancers including hepatocarcinogenesis is closely associated with diagnosis and prognosis. In hepatocellular carcinoma (HCC), malignancy level is related to the degree of histological differentiation., In order to establish a novel biomarker that can determine the degree of malignancy and forecast patient prognosis, we performed a microarray analysis to investigate the miRNA expression profiles in 110 HCC which were comprised of 60 moderately, 30 poorly, and 20 well differentiated HCC., We found that the expression of 12 miRNAs varied significantly according to the degree of histological differentiation. Particularly, miR-18b expression in poorly differentiated HCC was significantly higher than in well differentiated HCC. Based on miRanda and Targetscan target search algorithms and Argonaute 2 immunoprecipitation study, we noted that miR-18b can control the expression of trinucleotide repeat containing 6B (TNRC6B) as a target gene. Additionally, in two hepatoma cell lines, we found that over-expression of miR-18b or down-regulation of TNRC6B accelerated cell proliferation and loss of cell adhesion ability. Finally, we observed that after surgical resection, HCC patients with high miR-18b expression had a significantly shorter relapse-free period than those with low expression., miR-18b expression is an important marker of cell proliferation and cell adhesion, and is predictive of clinical outcome. From a clinical point of view, our study emphasizes miR-18b as a diagnostic and prognostic marker for HCC progression.
- PMID: 23496901
Expression Profile:
- Description:Over-expression of miR-18b is associated with the grade of malignancy of hepatocallular carcinoma
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE31164
- Platform: GPL10850
- Number of samples:110
- Overall design:In order to establish the novel biomarker for the degree of histological differentiation and forecasting relapse of HCC with HCV positive, we analyzed the miRNA expression profiles in 110 surgically resected hepatocellular carcinoma (60 moderately differentiated, 30 poorly differentiated, and 20 well differentiated HCC).
- Instrument:Agilent-021827 Human miRNA Microarray (V3) (miRBase release 12.0 miRNA ID version)
Design and Sample:
- Cancer Type:hepatocellular carcinoma
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:high grade vs low grade
- Case Sample:hepatocellular carcinoma poor differentiation
- Control Sample:hepatocellular carcinoma well differentiation
- Num of Case:30
- Num of Control:20
- Quantification Software:Limma
- Num of miRNAs:851
Identification:
- Num of Up:1
- Num of Down:4
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|