Detail
Experiment ID:
EXP00234
Reference:
- Title: miR-1 induces growth arrest and apoptosis in malignant mesothelioma.
- Author: Xu Y, Zheng M, Merritt RE, Shrager JB, Wakelee HA, Kratzke RA, Hoang CD
- Journal: Chest.2013 Nov ;144(5):1632-1643.doi:10.1378/chest.12-2770.
- Abstract: We investigated microRNA expression profiles of malignant pleural mesothelioma (MPM) specimens to identify novel microRNA that are potentially involved in the oncogenic transformation of human pleural cells., microRNA microarray transcriptional profiling studies of 25 MPM primary tumors were performed. We used normal pleural tissue from an unmatched patient cohort as normal comparators. To confirm microarray data, we used real-time quantitative polymerase chain reaction. Representative cell lines H513 and H2052 were used in functional analyses of miR-1., In addition to several novel MPM-associated microRNAs, we observed that the expression level of miR-1 was significantly lower in tumors as compared with normal pleural specimens. Subsequently, pre-miR of miR-1 was introduced into MPM cell lines to overexpress this microRNA. Phenotypic changes of these altered cells were assayed. The cellular proliferation rate was significantly inhibited after overexpression of miR-1. Early and late apoptosis was increased markedly in miR-1-transfected cell lines. Taken together, these data suggested that overexpression of miR-1 induced apoptosis in these MPM cell lines, acting as a tumor suppressor. We confirmed our observations by assessing in the transduced MPM cells cell cycle-related, proapoptotic, and antiapoptotic genes, which all showed coordinated, significant changes characteristic of the apoptotic phenotype., Further investigation and validation of our microRNA database of MPM may elucidate previously unrecognized molecular pathways and/or mechanisms by identifying novel microRNAs that are involved in malignant transformation. Our study has now found miR-1 to be one of these MPM-associated microRNAs, with potential pathogenic and therapeutic significance.
- PMID: 23828229
Expression Profile:
- Description:Human Mesothelioma MicroRNA Study Part 1
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE40345
- Platform: GPL8179
- Number of samples:31
- Overall design:MicroRNA microarray transcriptional profiling studies of 25 MPM primary tumors as well 6 normal pleural samples from an unmatched patient cohort as normal comparators were performed.
- Instrument:Illumina Human v2 MicroRNA expression beadchip
Design and Sample:
- Cancer Type:mesothelioma
- Cancer SubType:malignant pleural mesothelioma
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:malignant pleural mesothelioma
- Control Sample:normal tissue
- Num of Case:25
- Num of Control:6
- Quantification Software:Limma
- Num of miRNAs:846
Identification:
- Num of Up:97
- Num of Down:107
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|