Detail
Experiment ID:
EXP00236
Reference:
- Title: MicroRNA-regulated protein-protein interaction networks and their functions in breast cancer.
- Author: Lee CH, Kuo WH, Lin CC, Oyang YJ, Huang HC, Juan HF
- Journal: International journal of molecular sciences.2013 May 30;14(6):11560-606.doi:10.3390/ijms140611560.
- Abstract: MicroRNAs, which are small endogenous RNA regulators, have been associated with various types of cancer. Breast cancer is a major health threat for women worldwide. Many miRNAs were reported to be associated with the progression and carcinogenesis of breast cancer. In this study, we aimed to discover novel breast cancer-related miRNAs and to elucidate their functions. First, we identified confident miRNA-target pairs by combining data from miRNA target prediction databases and expression profiles of miRNA and mRNA. Then, miRNA-regulated protein interaction networks (PINs) were constructed with confident pairs and known interaction data in the human protein reference database (HPRD). Finally, the functions of miRNA-regulated PINs were elucidated by functional enrichment analysis. From the results, we identified some previously reported breast cancer-related miRNAs and functions of the PINs, e.g., miR-125b, miR-125a, miR-21, and miR-497. Some novel miRNAs without known association to breast cancer were also found, and the putative functions of their PINs were also elucidated. These include miR-139 and miR-383. Furthermore, we validated our results by receiver operating characteristic (ROC) curve analysis using our miRNA expression profile data, gene expression-based outcome for breast cancer online (GOBO) survival analysis, and a literature search. Our results may provide new insights for research in breast cancer-associated miRNAs.
- PMID: 23722663
Expression Profile:
- Description:MicroRNA expression profile of breast cancer patients with adjacent normal control group
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE45666
- Platform: GPL14767
- Number of samples:116
- Overall design:101 breast tumor + 15 adjacent breast normal tissue samples.
- Instrument:Agilent-021827 Human miRNA Microarray G4470C (Feature Number version)
Design and Sample:
- Cancer Type:breast cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:breast cancer
- Control Sample:normal breast
- Num of Case:101
- Num of Control:15
- Quantification Software:Limma
- Num of miRNAs:851
Identification:
- Num of Up:348
- Num of Down:242
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|