Detail
Experiment ID:
EXP00250
Reference:
- Title: Characterization of gene expression and activated signaling pathways in solid-pseudopapillary neoplasm of pancreas.
- Author: Park M, Kim M, Hwang D, Park M, Kim WK, Kim SK, Shin J, Park ES, Kang CM, Paik YK, Kim H
- Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.2014 Apr ;27(4):580-93.doi:10.1038/modpathol.2013.154.
- Abstract: Solid-pseudopapillary neoplasm is an uncommon pancreatic tumor with distinct clinicopathologic features. Solid-pseudopapillary neoplasms are characterized by mutations in exon 3 of CTNNB1. However, little is known about the gene and microRNA expression profiles of solid-pseudopapillary neoplasms. Thus, we sought to characterize solid-pseudopapillary neoplasm-specific gene expression and identify the signaling pathways activated in these tumors. Comparisons of gene expression in solid-pseudopapillary neoplasm to pancreatic ductal carcinomas, neuroendocrine tumors, and non-neoplastic pancreatic tissues identified solid-pseudopapillary neoplasm-specific mRNA and microRNA profiles. By analyzing 1686 (1119 upregulated and 567 downregulated) genes differentially expressed in solid-pseudopapillary neoplasm, we found that the Wnt/β-catenin, Hedgehog, and androgen receptor signaling pathways, as well as genes involved in epithelial mesenchymal transition, are activated in solid-pseudopapillary neoplasms. We validated these results experimentally by assessing the expression of β-catenin, WIF-1, GLI2, androgen receptor, and epithelial-mesenchymal transition-related markers with western blotting and immunohistochemistry. Our analysis also revealed 17 microRNAs, especially the miR-200 family and miR-192/215, closely associated with the upregulated genes associated with the three pathways activated in solid-pseudopapillary neoplasm and epithelial mesenchymal transition. Our results provide insight into the molecular mechanisms underlying solid-pseudopapillary neoplasm tumorigenesis and its characteristic less epithelial cell differentiation than the other common pancreatic tumors.
- PMID: 24072181
Expression Profile:
- Description:microRNA expression of pancreatic tumors
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE43796
- Platform: GPL15159
- Number of samples:31
- Overall design:To investigate the specific microRNA expression of SPN compared to other types of pancreatic tumor, we analyzed large-scale microRNA expressioin analysis to identify the molecular signature that may affect SPN tumorigenesis with mRNA expression profiles. Differentially expressed microRNAs were analyzed on SPNs, PCAs, NETs and Non-neoplastic tissues.
- Instrument:Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray 030840 (Probe Name version)
Design and Sample:
- Cancer Type:pancreatic cancer
- Cancer SubType:Solid-pseudopapillary neoplasm of pancreas
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:Solid-pseudopapillary neoplasm of pancreas
- Control Sample:normal pancreas
- Num of Case:13
- Num of Control:5
- Quantification Software:Limma
- Num of miRNAs:1205
Identification:
- Num of Up:144
- Num of Down:132
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
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