Detail
Experiment ID:
EXP00264
Reference:
- Title: Regulation of RAB5C is important for the growth inhibitory effects of MiR-509 in human precursor-B acute lymphoblastic leukemia.
- Author: Tan YS, Kim M, Kingsbury TJ, Civin CI, Cheng WC
- Journal: PloS one.2014;9(11):e111777.doi:10.1371/journal.pone.0111777.
- Abstract: "MicroRNAs (miRs) regulate essentially all cellular processes, but few miRs are known to inhibit growth of precursor-B acute lymphoblastic leukemias (B-ALLs). We identified miR-509 via a human genome-wide gain-of-function screen for miRs that inhibit growth of the NALM6 human B-ALL cell line. MiR-509-mediated inhibition of NALM6 growth was confirmed by 3 independent assays. Enforced miR-509 expression inhibited 2 of 2 additional B-ALL cell lines tested, but not 3 non-B-ALL leukemia cell lines. MiR-509-transduced NALM6 cells had reduced numbers of actively proliferating cells and increased numbers of cells undergoing apoptosis. Using miR target prediction algorithms and a filtering strategy, RAB5C was predicted as a potentially relevant target of miR-509. Enforced miR-509 expression in NALM6 cells reduced RAB5C mRNA and protein levels, and RAB5C was demonstrated to be a direct target of miR-509. Knockdown of RAB5C in NALM6 cells recapitulated the growth inhibitory effects of miR-509. Co-expression of the RAB5C open reading frame without its 3 untranslated region (3UTR) blocked the growth-inhibitory effect mediated by miR-509. These findings establish RAB5C as a target of miR-509 and an important regulator of B-ALL cell growth with potential as a therapeutic target."
- PMID: 25368993
Expression Profile:
- Description:miRNA expression data from normal and Malignant hematopoietic cells
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE51908
- Platform: GPL8786
- Number of samples:190
- Overall design:AML cell lines and patient samples, B ALL cell lines and Patient samplest, T ALL cell lines and patient samples, norma B cells, normal granulocytes, normal monocytes, normal T cells and normal CD34+ cells were used for RNA extraction and hybridization on Affymetrix microarrays. All the AML, B ALL, T ALL cell lines were cultured in vitro under appropriate culture conditons and harvested in their log phase growth for RNA extraction. AML, B ALL, and T ALL patient samples were collected...(I assume these are the PBMCs from eitehr peripheral blood or bone marrow from patients, please confirm). Normal B cells, granulocytes, monocytes, and T cells were purified from human peripheral blood of normal healthy donors.
- Instrument:[miRNA-1] Affymetrix Multispecies miRNA-1 Array
Design and Sample:
- Cancer Type:leukemia
- Cancer SubType:T acute lymphoblastic leukemia
- Cell Line:N/A
- Experimental Design:cancer vs normal
- Case Sample:T acute lymphoblastic leukemia
- Control Sample:T cell
- Num of Case:13
- Num of Control:17
- Quantification Software:Limma
- Num of miRNAs:597
Identification:
- Num of Up:69
- Num of Down:54
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|